安徽农业科学2018，Vol.46Issue(15)：72-74,3.
周龄·初始注射时间对小鼠超数排卵的影响
The Influence of Week-age and Initial Injection Time on Superovulation in Mice
巩雪洁 1臧敦安 1许文豪1
作者信息
- 1. 中南民族大学生命科学学院医学生物研究所,湖北武汉430074
- 折叠
摘要
Abstract
[Objective]To discuss the influence of week-age and initial injection time on superovulation in mice.[Method]The C57BL/6J mice were treated with 10 IU PMSG+10 IU HCG interval 48h,and the number of zygotes was collected and counted.[Result]The effect of the four week age superovulation was better than that of eight weeks,and it could reach 26.86.The time of PMSG and HCG injection of C57BL/6J mice at 4 weeks old was 12-13 at noon,and the number of zygotes collected was up to 30 and in vitro culture of the fertilized ovum was in good condi-tion.[Conclusion]The 4-week old C57BL/6J mice are suitable for superovulation.When the injection time of PMSG and HCG is 12-13 at noon, the superovulation effect is relatively ideal.关键词
小鼠/超排卵/激素/周龄/初始注射时间Key words
Mouse/Superovulation/Hormone/Week-age/Initial injection time分类
农业科技引用本文复制引用
巩雪洁,臧敦安,许文豪..周龄·初始注射时间对小鼠超数排卵的影响[J].安徽农业科学,2018,46(15):72-74,3.
