安徽农业科学2018，Vol.46Issue(15)：87-88,2.
降低软枣猕猴桃初代培养污染率的研究
Study on the Reduction of Contamination Rate of Actinidia arguta Planch.Primary Culture
摘要
Abstract
[Objective]To screen the most suitable treatment for reducing Actinidia arguta primary culture contamination rate and increasing ex-plant induction rate.[Method]Semi-woody shoots were used as explants to screen the most suitable treatment for reducing A.arguta primary cul-ture contamination rate and increasing explant induction rate by combining sterilization time and ampicillin medium concentration.[Result]In-creasing the sterilization time to 10 s could reduce the contamination rate of A.arguta primary culture,and the contamination rate of primary cul-ture decreased with the increasing of ampicillin concentration during 100~150 mg/L.Increasing explant sterilization time and ampicillin concen-tration in culture medium could increase the induction rate of A.arguta.[Conclusion]1/2MS+100 mg/L ampicillin,1/2MS+150 mg/L ampi-cillin, explants were sterilized with 75% alcohol with 10 s+0.1% mercuric chloride for 8 min and explants 75% alcohol sterilization 10 s+0.1% mercury chloride sterilization 10 min are the suitable methods with low contamination rate and high induction rate.关键词
软枣猕猴桃/污染率/灭菌时间/氨苄青霉素Key words
Actinidia arguta Planch./Contamination rate/Sterilization time/Ampicillin分类
农业科技引用本文复制引用
孙书伟,姚平,宋洁,周文杰..降低软枣猕猴桃初代培养污染率的研究[J].安徽农业科学,2018,46(15):87-88,2.基金项目
辽东学院农业攻关项目(2016YY013). （2016YY013）
