北京中医药大学学报2018，Vol.41Issue(5)：372-375,4.DOI:10.3969/j.issn.1006-2157.2018.05.004
济阴颗粒对围绝经期综合征大鼠卵巢Caspase依赖性细胞凋亡通路的影响
Influence of Jiyin Capsule on apoptosis pathway of ovarian caspase de-pendent cells in rats with peri-menopausal syndrome
摘要
Abstract
Objective To discuss the effective mechanism of Jiyin(Nourishing Yin)Capsule in interve-ning peri-menopausal syndrome.Methods Normal rats(n=10)were randomly chosen into blank group. The model of peri-menopausal syndrome was established with intraperitoneal injection of VCD once a day for 60 d.When estrous cycle was disorder,level of serum estradiol(E2)was lower than the lowest level of that in blank group and level of follicle-stimulating hormone(FSH)was higher than the highest level of that in blank group, the modeling was success.The modeled rats were randomly divided into 6 groups:model group,progynova group(PGV group),Kunbao(Female Treasure)Pill group(KBP group), and low-dose,mid-dose and high-dose Jiyin Capsule groups(each n=10).After grouping,blank group and model group were given distilled water,and other groups were given corresponding medicinals once a day for 30 d.The mRNA expressions of caspase-3,caspase-8,caspase-9 and Apaf-1 were detected by using re-al-time fluorescence quantitative polymerase chain reaction(RT-PCR).Results Compared with blank group,mRNA expressions of caspase-3,caspase-8,caspase-9 and Apaf-1 increased significantly in model group(P<0.01).Compared with model group, mRNA expressions of caspase-3, caspase-8, caspase-9 and Apaf-1 decreased significantly in Jiyin Capsule groups.Conclusion Jiyin Capsule can inhibit cell ap-optosis of ovarian through down-regulating conduction of apoptosis pathway of caspase dependent cells, which maybe one of its effective mechanisms of intervening peri-menopausal syndrome.关键词
济阴颗粒/围绝经期综合征/Caspase依赖性细胞凋亡通路/大鼠Key words
Jiyin(Nourishing Yin)Capsule/peri-menopausal syndrome/apoptosis pathway of caspase dependent cells/rats分类
医药卫生引用本文复制引用
赵磊,黄赫,慕杨娜,袁媛,包玉龙,梁茂新..济阴颗粒对围绝经期综合征大鼠卵巢Caspase依赖性细胞凋亡通路的影响[J].北京中医药大学学报,2018,41(5):372-375,4.基金项目
"十二五"国家重大新药创制资助项目(No.2012zx09303017),国家临床重点专科建设资助项目,国家中医药管理局临床中药学重点学科资助项目 National New Drugs Development of the Twelfth Five-year Plan(No.2012zx09303017),National Clinical Key Specialty Construction,Clinical Chinese Pharmacology Key Discipline of the State Administration of Traditional Chinese Medicine （No.2012zx09303017）
