北京中医药大学学报2018，Vol.41Issue(5)：383-394,12.DOI:10.3969/j.issn.1006-2157.2018.05.006
沙棘酚类特征成分含量测定及其特征图谱质量表征关联分析研究
Quantitative determination of phenolic characteristic components and their quality representation and correlation analysis of specific chromato-grams in Shaji
摘要
Abstract
Objective To establish an analysis method for quantitatively determining phenolic character-istic components and correlation analyzing their quality representation of specific chromatograms in Shaji (Seabuckthorn Fruit,Hippophae rhamnoides),and to review the quality of Shaji effectively and accurate-ly by applying association analysis-reviewing mode.Methods HPLC-PDA method was used to quantita-tively determine the content of phenolic characteristic components in 11 batches of Shaji(protocatechuic acid,ellagic acid,narcissin,quercetin,isorhamnetin),and to establish phenolic specific chromatograms of Shaji.The quality of 11 batches of Shaji was characterized based on teasing characteristic peaks and chemical types.The quantity of 11 batches of Shaji was characterized based on quantity and peak areas of protocatechuic acid, ellagic acid, narcissin, quercetin, isorhamnetin, phenolic acids(represented by protocatechuic acid)and flavonoids(represented by narcissin)in the specific chromatograms.The characterized results of quality and quantity of 11 batches of Shaji were given association analysis based on baseline material of Shaji.Results The characteristic components of protocatechuic acid, ellagic acid,narcissin,quercetin,isorhamnetin all had good linear correlation,and the results of methodological investigation were in accordance with the quantitative determination requirements.Taken batch 7 of Shaji as baseline material, there were totally 18 characteristic peaks in phenolic specific chromatograms of Shaji,including 3 peaks of phenolic acids and 15 peaks of flavonoids, and all 18 characteristic peaks appeared in the chromatograms of 11 batches of Shaji.The quantity of characteristic components were higher in batch 8, 7, 10, 4, 5, 11 and 1 after analyzed and reviewed by using association analysis-reviewing mode.The relevance of batch 6, 2, 3, 1 and 11 was the highest with baseline material of Shaji.Comprehensive reviewing showed that the excellent extents of batch 1,11,8,6 and 7 were prior. Conclusion The quantitative determination method of phenolic characteristic components in Shaji estab-lished in this study is easy and accurate.The association analysis-reviewing mode for quality characteriza-tion of phenolic specific chromatograms can be used for analyzing the quality and application validity of Shaji and reviewing quality of Shaji effectively and accurately.关键词
沙棘/酚类/特征图谱/关联分析/质量评价Key words
Shaji(Seabuckthorn Fruit/Hippophae rhamnoides)/phenols/specific chromatograms/cor-relation analysis/quality review分类
医药卫生引用本文复制引用
张元元,王梦林,范圆圆,冯鑫,李思潼,黄玉娟,姜艳艳,石任兵,吕珊,陈梦杰,孔静,孙仁弟,张媛媛,路东波,武秋红,李文霞..沙棘酚类特征成分含量测定及其特征图谱质量表征关联分析研究[J].北京中医药大学学报,2018,41(5):383-394,12.基金项目
"十二五"国家科技支撑计划资助项目(No.2012BAI29B06),北京市教委共建科研基地建设资助项目,北京中医药大学重点学科开放课题资助项目(No.2013-2DXKKF-23)National Sci-Tech Plan of the 12th Five-Year Plan(No.2012BAI29B06) （No.2012BAI29B06）
