北京中医药大学学报2018，Vol.41Issue(5)：400-404,5.DOI:10.3969/j.issn.1006-2157.2018.05.008
地骨皮中咖啡酰丁二胺、地骨皮乙素和绿原酸含量测定方法研究
Quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi
摘要
Abstract
Objective To establish a method for quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi(Chinese Wolfberry Root-bark,Cortex Lycii).Methods The preparation method for solution of test sample was established by using single-factor investigation. HPLC procedure was performed for determining the content of N-caffeoylputrescine, kukoamine B and chlorogenic acid on chromatographic column of Waters Xbridge C 18(4.6 mm×250 mm,5 μm),mobile phase was methanol -0.05%trifluoroacetic acid in gradient elution.The flow velocity was 1.0 mL/min and the detection wavelength was 290 nm at 30℃.Results The solution of test sample was prepared af-ter Digupi was extracted with 100 times amount of 70% methanol by using reflux extraction for 60 min. N-caffeoylputrescine showed a good linear relationship at a range from 0.013 75 mg/mL to 0.220 0 g/L (r=0.999 5),and average recovery was 99.25%(RSD=1.81%, n=6).Kukoamine B showed a good linear relationship at a range from 0.057 875 g/L to 0.926 0 mg/mL(r=0.999 8), and average recovery was 101.01%(RSD=2.81%,n=6).Chlorogenic acid showed a good linear relationship at a range from 0.009 25 g/L to 0.148 0 g/L(r=0.999 5), and average recovery was 99.51%(RSD=2.32%,n=6).Conclusion The established quantitative determination method is easy and accurate with a good repeatability,which can lay a foundation for the quality control and reviewing of Digupi.关键词
地骨皮/高效液相色谱法/含量测定/咖啡酰丁二胺/地骨皮乙素/绿原酸Key words
Digupi(Chinese Wolfberry Root-bark/Cortex Lycii)/high performance liquid chromato-graph/quantitative determination/N-caffeoylputrescine/kukoamine B分类
医药卫生引用本文复制引用
苏磊,姜艳艳,陈路晓,刘静,刘斌..地骨皮中咖啡酰丁二胺、地骨皮乙素和绿原酸含量测定方法研究[J].北京中医药大学学报,2018,41(5):400-404,5.基金项目
国家自然科学基金资助项目(No.81173520),北京中医药大学研究生自主选题资助项目(No.2017-JYB-XS-142)National Natural Science Foundation of China(No.81173520) （No.81173520）
