南方医科大学学报2017，Vol.37Issue(3)：301-307,7.DOI:10.3969/j.issn.1673-4254.2017.03.04
基于数字全息显微术的登革病毒感染C6/36细胞的3D形态学
Three-dimensional morphology of C6/36 cells infected by dengue virus: a study based on digital holographic microscopy
摘要
Abstract
Objective To monitor the 3-dimensional (3D) morphological changes of C6/36 cells during dengue virus (DENV) infection using a live-cell imaging technique based on digital holographic microscopy and provide clues for better understanding the mechanisms of DENV infection.Methods C6/36 cells were seeded in 6-well plates to determine the optimal imaging density under a holographic cell imager,and the morphological changes of the cells were recorded in response to a culture temperature change from 28 ℃ to 37 ℃.C6/36 cells were infected with 4 DENV strains with different serotypes at 28 ℃ and incubated at 37 ℃ for 24 h,and the 3D holograms and relevant morphological parameters were recorded at different time points using HoloMonitor M4 holographic cell imaging and analysis system.Results The holograms of C6/36 cells inoculated at the optimal density for imaging (4 × 105 per well) showed unified 3D morphologies of the single cells with minimal dispersions in the cell area,thickness and volume (P＜0.05),which did not undergo obvious changes when the cells were incubated at 37 ℃ for 24 h (P＞0.05).The cell area and volume of the cells infected with the 4 DENV strains all increased and the cell thickness was reduced during incubation.Among the 4 strains,DENV-1 and DENV-2 caused reduced cell thickness while DENV-3 and DENV-4 increased the cell thickness,and the pattern and degree of such changes differ among the 4 strains.Conclusions Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection.The 4 DENV strains with different serotypes causes characteristic cell damages during infection.关键词
登革病毒/数字全息显微术/3D形态学Key words
dengue virus/digital holographic microscopy/3D morphology引用本文复制引用
余健海,刘旭玲,刘雨菁,何晓恩,惠媛,张宝,朱利,赵卫..基于数字全息显微术的登革病毒感染C6/36细胞的3D形态学[J].南方医科大学学报,2017,37(3):301-307,7.基金项目
国家自然科学基金(31270974,31470271) （31270974,31470271）
NSFC-广东联合基金(U1132002) （U1132002）
广东省及广州市防控登革热应急科技攻关项目(2013A020229004,201508020263)Supported by National Natural Science Foundation of China (31270974,31470271). （2013A020229004,201508020263）
