南方医科大学学报2017，Vol.37Issue(3)：354-359,6.DOI:10.3969/j.issn.1673-4254.2017.03.13
miR-501-5p在宣威地区肺腺癌组织中的表达及临床意义
Expression profile of miR-501-5p in lung adenocarcinoma patients from Xuanwei area
摘要
Abstract
Objective To investigate the relationship between miR-501-5p expression and the clinicopathological factors in patients with lung adenocarcinoma in Xuanwei area.Methods Surgical specimens of lung adenocarcinoma and paired adjacent tissues from 24 patients with lung adenocarcinoma from Xuanwei area were examined for miR-501-5p expressionusing microRNA microarray technique and qPCR.Chi-square test was used to analyze the association of miR-501-5P expression with the clinicopathological characteristics of the patients.Multiple regression analysis was performed to analyze the association of miR-501-5p expression with the patients' gender,age,tumor stage,and preoperative CEA level.Results MicroRNA microarray analysis and qPCR validation results revealed significantly upregulated expressions of miR-501-5p in patients with lung adenocarcinoma from Xuanwei area (P＜0.01).The microarray data showed an up-regulation of miR-501-5p by 3.17 folds in lung adenocarcinoma tissue compared with the adjacent tissue (P=0.22376,FDR=0.071395).Chi-square test indicated that miR-501-5p expression level was associated with the patients' age (f=7.168,P=0.014),TNM stage (f=36.627,P＜0.01),and preoperative serum CEA level (f=30.045,P＜0.01),but not with the patients' gender (f=3.612,P=0.071).Multiple regression analysis revealed that miR-501-5p expression was positively correlated with the patients' age,TNM stage of the tumor,and serum CEA (P＜0.05).Conclusion miR-501-5p expression is up-regulated in lung adenocarcinoma with significant associations with the patients' age,TNM stages and serum CEA level in patients from Xuanwei area,suggesting its potential role in the tumorigenesis and progression of lung adenocarcinoma in Xuanwei area.关键词
microRNAs/肺癌/miR-501-5pKey words
microRNAs/lung adenocarcinoma/miR-501-5p引用本文复制引用
陈帅,黄云超,周永春,陈颖,陈小波,李光剑,雷玉洁,田林玮,赵光强,黄秋博..miR-501-5p在宣威地区肺腺癌组织中的表达及临床意义[J].南方医科大学学报,2017,37(3):354-359,6.基金项目
国家自然科学基金(U1202224,81460441) （U1202224,81460441）
云南省应用基础研究面上项目(2016FB145) （2016FB145）
云南省卫生科技计划项目(2014NS001) （2014NS001）
云南省医疗卫生单位内设研究基金(2016NS106) （2016NS106）
云南省肿瘤医院博士科研启动基金(BSJJ201502)Supported by National Natural Science Foundation of China (U1202224,81460441). （BSJJ201502）
