南方医科大学学报2017，Vol.37Issue(5)：594-599,6.DOI:10.3969/j.issn.1673-4254.2017.05.05
FABP7在小鼠胎盘组织及人滋养层细胞HTR-8/Svneo中的表达
Expression of FABP7 in mouse placenta tissue and human trophoblast HTR-8/Svneo cells
摘要
Abstract
Objective To detect the expression of FABP7 in the placenta of pregnant mice and in HTR-8/Svneo cells.Methods Real-time PCR and immunofluorescence were used to detect FABP7 mRNA and protein expressions in the uterine and placental tissue of pregnant mice at different days of gestation.FABP7 expression was also detected in cultured HTR-8/Svneo cells using immunofluorescence assay.The mice were treated with E2,P4 or their combination for 6 and 24 h and Fabp7 mRNA level in the uterus was detected with real-time PCR.Results At 7.5-10.5 days of gestation,the pregnant mice showed,positive expressions of Fabp7 mRNA in the uterus and placenta,and FABP7 protein was detected in the decidualized cells and trophoblast giant cells.The expressions of FABP7 were detected at both the mRNA and protein levels in cultured HTR-8/Svneo cells.In mice treated with P4 alone or with E2+P4 for 6 and 24 h,the expression level of Fabp7 mRNA was upregulated in the uterus.Fabp7 upregulation was observed in mice at 24 h following E2 treatment but not at 6 h.Conclusion FABP7 is expressed in trophoblast giant cells and decidual cells in the placental tissue of mice and in cultured HTR-8/Svneo cells,suggesting the involvement of FABP7 in placental development and in maintenance of pregnancy.E2 and P4 can regulate the expression of FABP7 in mouse uterus.关键词
FABP7/子宫/HTR-8/Svneo细胞/胎盘Key words
FABP7/uterus/HTR-8/Svneo/placenta引用本文复制引用
田鎏,廖晖淇,杨慧,马妮,张昌军,刁红录..FABP7在小鼠胎盘组织及人滋养层细胞HTR-8/Svneo中的表达[J].南方医科大学学报,2017,37(5):594-599,6.基金项目
国家自然科学基金(31671565) （31671565）
湖北医药学院创新团队(2014CXX03,FDFR201604) （2014CXX03,FDFR201604）
湖北医药学院重点学科建设项目资助项目Supported by National Natural Science Foundation of China (31671565). （31671565）
