南方医科大学学报2017，Vol.37Issue(5)：607-613,7.DOI:10.3969/j.issn.1673-4254.2017.05.07
抗坏血酸/聚已酸内酯生物材料修复新西兰白兔软骨缺损
Effect of polycaprolactone-ascobic acid scaffold in repairing articular cartilage defects in rabbits
摘要
Abstract
Objective To investigate the effect of polycaprolactone-ascobic acid (PCL-AA) scaffolds in promoting repair of articular cartilage defects in a rabbit model.Methods The cartilage defects (3.5 mm in diameter and 3.0 mm in depth) were created in the trochlear groove of the bilateral knees of eight 6-month-old male New Zealand white rabbits.The rabbit models were then randomized into 3 groups to receive implantation of PCL-AA scaffolds (group A,n=8),implantation of PCL scaffolds without AA (group B,n=5),or no treatment (group C,n=3).In groups A and B,the mixture of fibrin gel (10 μg) and thrombinogen (10 μg) was injected into the defects to fix the scaffolds during the surgery.Histological analyses and quantitative assessments of defect repair were conducted at 6 and 12 weeks after implantation of the scaffold.Results At 6 weeks after scaffold implantation,macroscopic observation showed better filling of the cartilage defects in group A than in group B,while no obvious defect repair was observed in group C.The rabbits in group A showed a significant improvement of the Wakitani score than those in group B (4.05±1.11 vs 7.05±0.98,P＜0.05).HE staining revealed the presence of newly generated cells in and around the PCL-AA scaffolds without inflammatory cells.Safranin O staining showed a significantly greater ECM of the newly regenerated tissue in groups A and B than in group C (P＜0.05),and the volume of the regenerated cartilage and cells was significantly greater in group A than in group B (P＜0.05).Samples harvested at 12 weeks showed more hyalione-like cartilage formation than that at 6 weeks in group A.Conclusion PCL-AA scaffolds have a good biocompatibility and promotes the healing of articular cartilage defects.Adding ascorbic acid into PCL scaffolds better promotes cartilage formation in terms of both quantity and quality of the regenerated tissues.PCL-AA scaffolds can serve as a promising biomaterial to promote the regeneration of articular cartilage using tissue engineering techniques.关键词
软骨组织工程/聚已内酯/抗坏血酸/生物材料/关节软骨再生Key words
cartilage tissue engineering/polycaprolactone/ascorbic acid/biomaterial/articular cartilage regeneration引用本文复制引用
黄志会,宋兵,陈宇璠,廖哲霆,赵亮..抗坏血酸/聚已酸内酯生物材料修复新西兰白兔软骨缺损[J].南方医科大学学报,2017,37(5):607-613,7.基金项目
国家自然科学基金(31328008) （31328008）
广东省自然科学基金(2014A030313275) （2014A030313275）
广东省科学计划项目(2012B010200024) （2012B010200024）
广州市“珠江科技新星”人才培育计划(2012027)Supported by National Natural Science Foundation of China (31328008). （2012027）
