南方医科大学学报2017，Vol.37Issue(5)：668-672,5.DOI:10.3969/j.issn.1673-4254.2017.05.17
3D打印明胶海藻酸钠凝胶支架对人牙髓细胞的黏附增殖作用
Gelatin/alginate hydrogel scaffolds prepared by 3D bioprinting promotes cell adhesion and proliferation of human dental pulp cells in vitro
摘要
Abstract
Objective To evaluate the cytotoxicity of gelatin/alginate hydrogel scaffolds prepared by 3D bioprinting in human dental pulp cells (HDPCs) and compare the cell adhesion and proliferation of the cells seeded in the biomaterial using two different methods.Methods HDPCs isolated by tissue block culture and enzyme digestion were cultured and passaged.Gelatin/alginate hydrogel scaffolds were printed using a bioplotter,and the cytotoxicity of the aqueous extracts of the scaffold material was tested in the third passage of HDPCs using cell counting kit-8.Scanning electron microscopy and trypan blue were used to assess the adhesion and proliferation of the cells seeded in the scaffold material at a low or high concentration.Results The aqueous extract of the scaffolds at different concentrations showed no obvious cytotoxicity and promoted the proliferation of HDPCs.The scaffolds had a good biocompatibility and HDPCs seeded in the scaffold showed good cell growth.Cell seeding at a high concentration in the scaffold better promoted the adhesion of HDPCs and resulted in a greater cell number on the scaffold surface compared with low-concentration cell seeding after a 5-day culture (P＜0.05).Conclusion Gelatin/alginate hydrogel scaffolds prepared by 3D bioprinting has a good biocompatibility and promotes the proliferation of HDPCs,and can be used as a scaffold material for tooth regeneration.Cell seeding at a high concentration can better promote cell adhesion to the scaffold material.关键词
3D打印明胶海藻酸钠/人牙髓细胞/细胞毒性/黏附/增殖Key words
3D bioprinting/gelatin/alginate/human dental pulp cells/cytotoxicity/adhesion/proliferation引用本文复制引用
于海悦,麻丹丹,吴补领..3D打印明胶海藻酸钠凝胶支架对人牙髓细胞的黏附增殖作用[J].南方医科大学学报,2017,37(5):668-672,5.基金项目
国家自然科学基金青年项目(81400495) （81400495）
广东省自然科学基金博士启动项目(2015A3030310101) Supported by National Natural Science Foundation of China (81400495). （2015A3030310101）
