南方医科大学学报2018，Vol.38Issue(5)：534-540,7.
可诱导共刺激分子介导的Th17细胞极化对自发性高血压大鼠肾纤维化的影响
Role of inducible costimulatory molecule-mediated Th17 cell polarization in renal fibrosis in spontaneously hypertensive rats
摘要
Abstract
Objective To explore the role of inducible costimulatory molecule (ICOS) signaling pathway-mediated Th17 cells polarization in renal damage in essential hypertension.Methods Four-week-old spontaneously hypertensive rats(SHR)were randomly divided into control (SHR-C) group and intervention (SHR-I) group and subjected to intraperitoneal injections of PBS and ICOS monoclonal antibody for 2 weeks,respectively.Blood pressure of the rats was monitored using noninvasive tail artery blood pressure measuring instrument. The percentage of Th17 cells in the splenocytes was analyzed using flow cytometry,and the expression levels of IL-17A mRNA in the rat's kidneys were detected using RT-PCR.The levels of IL-17A and TGF-β1 in the plasma and kidneys were dynamically detected using ELISA and immunohistochemistry, respectively. Renal pathological changes in the rats were detected using Masson staining.Results At the age of 10 and 30 weeks,the rats in SHR-C group had a significantly higher blood pressure than those in SHR-I group(P<0.05 or 0.01).In rats in SHR-C group, Th17 cells percentage in the splenocytes and IL-17A mRNA level in the kidney was significantly higher than those in SHR-I group from the age of 6 weeks(P<0.05).The expressions of IL-17A and TGF-β1 in the plasma and kidney were significantly higher in SHR-C group than that in SHR-I group at 6 weeks(P<0.05).Compared with those in SHR-C group,the rats in SHR-I group showed significant alleviation of renal fibrosis from the age of 30 weeks (P<0.05). Conclusion The ICOS signaling pathway-mediated Th17 cells polarization plays an important role in renal fibrosis in hypertensive rats.关键词
可诱导共刺激分子/Th17细胞/自发性高血压大鼠/肾纤维化Key words
inducible costimulatory molecule/Th17 cells/spontaneously hypertensive rats/renal fibrosis引用本文复制引用
王瑜,王博士,惠旭,乔君,李委振,孙楠..可诱导共刺激分子介导的Th17细胞极化对自发性高血压大鼠肾纤维化的影响[J].南方医科大学学报,2018,38(5):534-540,7.基金项目
安徽高校省级自然科学重点研究项目(KJ2017A098) （KJ2017A098）
安徽理工大学博士科研基金(11046) （11046）
安徽理工大学中青年学术骨干培养工程(10167) （10167）
