南方医科大学学报2018，Vol.38Issue(5)：541-546,6.
氯化锂通过AKT/GSK-3β阻滞近端肾小管上皮细胞周期在G2期
Lithium chloride arrests HK-2 cell cycle in G2 phase through AKT/GSK-3β signal pathway
摘要
Abstract
Objective To investigate the effect of lithium chloride (LiCl) on cell cycle of HK-2 cells and explore the possible pathways involved.Methods HK-2 cells were treated with LiCl at different concentrations(5,12.5,20,and 25 mmol/L)for 12, 24,48,or 72 h,and the changes in cell cycle and viability were detected using flow cytometry and CCK-8 assay,respectively. Western blotting was used to analyze the changes in the expressions of cyclin B1 and CDK1 (the two G2 phase-related proteins)and those of AKT/GSK-3β signaling pathway-related proteins in the treated cells.Results LiCl treatment time-and concentration-dependently increased HK-2 cell percentage in G2 phase and decreased the cell vitality. The expressions of cyclin B1,CDK1,p-GSK-3β,and β-catenin increased and the expression of p-AKT decreased significantly in the cells as LiCl treatment time and concentration increased.Conclusion LiCl may cause HK-2 cell cycle arrest in G2 phase through activation of the AKT/GSK-3β signaling pathway.关键词
氯化锂/AKT/GSK-3β/HK-2细胞/G2期Key words
lithium chloride/AKT/GSK-3β/HK-2 cells/cell cycle引用本文复制引用
姜婷婷,张雯英,向晓红,束双双,谢唯,汤珣,章俊..氯化锂通过AKT/GSK-3β阻滞近端肾小管上皮细胞周期在G2期[J].南方医科大学学报,2018,38(5):541-546,6.基金项目
国家自然科学基金(81202842) （81202842）
广州市科技计划项目(11C22120703) （11C22120703）
广东省科技计划项目(2013B021800149)Supported by National Natural Science Foundation of China(81202842). （2013B021800149）
