南方医科大学学报2018，Vol.38Issue(5)：547-553,7.
线粒体通透性转换孔在内吗啡肽-1后处理减轻大鼠心肌缺血再灌注损伤中的作用
Role of mitochondrial permeability transition pore in mediating the effect of endomorphin-1 postconditioning against myocardial ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate the role of mitochondrial permeability transition pore (MPTP) opening in mediating the effect of endomorphine-1 postconditioning to alleviate myocardial ischemia-reperfusion(IR)injury in rats.Methods Forty-five male SD rats were randomized equally for sham operation,myocardial IR injury,endomorphin-1 postconditioning,atractyloside (a MPTP opener)postconditioning,or endomorphin-1+atractyloside postconditioning.The hemodynamic param-eters of the rats were monitored in real time via carotid artery cannulation to the left ventricle.After reperfusion, plasma samples were collected for biochemical analyses.The size of myocardial infarct area was detected using Evans blue and TTC double staining, and the myocardial expressions of apoptosis-related proteins Bax,Bcl-2 and cleaved caspase-3 were analyzed using Western blotting. Results Myocardial IR injury resulted in significantly decreased heart rate and blood pressure in the rats (P<0.05). Compared with those in IR group, the rats with endomorphin-1 postconditioning showed significantly increased heart rate and blood pressure(P<0.05),lowered contents or activities of LDH,CK-MB,cTnI,IL-6,TNF-α,Cyt-C and MDA in the plasma (P<0.05), increased plasma SOD activity (P<0.05), reduced size of myocardial infarction, decreased myocardial expression of Bax and cleaved caspase-3 protein(P<0.05),and increased myocardial expression of Bcl-2 protein(P<0.05).All these changes induced by endomorphin-1 were obviously reversed by atractyloside postconditioning(P<0.05).Conclusion Endomorphin-1 postconditioning protects against myocardial IR injury in rats probably by inhibiting the opening of MPTP and reducing cardiac myocyte apoptosis via down-regulating cleaved caspase-3 expression.关键词
心肌缺血再灌注损伤/线粒体通透性转换孔/苍术苷/内吗啡肽-1后处理Key words
myocardial ischemia/reperfusion injury/mitochondrial permeability transition pore/atractyloside/endomorphin-1 postconditioning引用本文复制引用
黄艳平,杨天华,金植炎,王娅,叶红伟,高琴,李正红..线粒体通透性转换孔在内吗啡肽-1后处理减轻大鼠心肌缺血再灌注损伤中的作用[J].南方医科大学学报,2018,38(5):547-553,7.基金项目
国家自然科学基金(81472656,81770297) （81472656,81770297）
安徽省自然科学基金(1508085MH169,1508085MH170) （1508085MH169,1508085MH170）
蚌埠医学院研究生科研创新项目(Byycx1707)Supported by National Natural Science Foundation of China(81472656,81770297). （Byycx1707）
