南方医科大学学报2018，Vol.38Issue(5)：625-629,5.
日本血吸虫半胱氨酸蛋白酶抑制剂干预脂多糖诱导的小鼠脓毒症
Intervention with Schistosoma japonicum cysteine protease inhibitor for treatment of lipopolysaccharide-induced sepsis in mice
摘要
Abstract
Objective To observe the effect of Schistosoma japonicum cysteine protease inhibitor (r SjCystatin) for treatment of lipopolysaccharide(LPS)-induced sepsis in mice.Methods After a week of adaptive feeding,54 BALB/c mice were randomly divided into normal control group(group A),sepsis group(group B),and rSjCystatin intervention group(group C).The mice in group A received an intraperitoneal injection of PBS(100 μL),and those in groups B and C were injected with PBS(100 μL) containing LPS(10 mg/kg);the mice in group C were also intraperitoneally injected with 25 μg sjCystatin in 100 μL PBS 30 min after LPS injection.From each group,10 mice were randomly selected 24 h after PBS or LPS injection for detecting serum levels of TNF-α, IL-6, and IL-10 using ELISA and the levels of ALT,AST, BUN, and Cr using automatic biochemical analyzer; the pathological changes in the liver,lung and kidney were observed with HE staining.The remaining 8 mice in each group were used for observing the changes in the general condition and the 72-h survival.Results The 72-h survival rates of the mice was 100% in group A,0 in group B,and 36% in group C,showing a significant difference among the 3 groups(P<0.05).Compared with those in group A,the mice in group B exhibited obvious liver,lung,and renal pathologies with increased levels of ALT, AST, BUN, Cr, IL-6, and TNF-α (P<0.05). Treatment with sjCystatin significantly lessened LPS-induced organ pathologies, lowered the levels of liver and renal functional indexes and the pro-inflammatory cytokines,and increased the serum level of IL-10 in the mice(P<0.05).Conclusion SjCystatin can produce a significant therapeutic effect on sepsis induced by LPS in mice.关键词
脓毒症/日本血吸虫半胱氨酸蛋白酶抑制剂/脂多糖/促炎因子/免疫调节因子Key words
sepsis/Schistosoma japonicum cysteine protease inhibitors/lipopolysaccharide/proinflammatory cytokines/immunoregulatory cytokines引用本文复制引用
万勇坤,张敏,钱海春,杨芳芳,谢红,高世芳,方强,杨小迪,刘牧林,李徽徽,左琳,王小莉,王黎源,贺文欣,姜辉,王守祥,盛洁..日本血吸虫半胱氨酸蛋白酶抑制剂干预脂多糖诱导的小鼠脓毒症[J].南方医科大学学报,2018,38(5):625-629,5.基金项目
安徽省自然科学基金(1508085QH158) （1508085QH158）
安徽高校科研创新平台团队项目(2016-40) （2016-40）
安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016159) （gxyqZD2016159）
安徽省高等学校自然科学研究重点项目(KJ2016A708、KJ2017A235) （KJ2016A708、KJ2017A235）
国家及省级大学生创新创业训练计划项目(201610367009,201610367029) （201610367009,201610367029）
安徽省人社厅科研课题(2016H105) （2016H105）
蚌埠医学院自然科学基金重点项目(BYKY1624ZD,BYKY1648) （BYKY1624ZD,BYKY1648）
