中国耳鼻咽喉头颈外科2017，Vol.24Issue(12)：620-624,5.DOI:10.16066/j.1672-7002.2017.12.005
叶酸缺乏导致小鼠听力障碍机制的实验研究
Experimental research of mechanism of hearing impairment cause by lack of folic acid in mouse
摘要
Abstract
OBJECTIVE To clarify the influence factors of hearing impairment in mice caused by lack of folic acid and the mechanism.METHODS Mice were randomly divided into 2 groups,fed with no folic acid diet and normal diet for 10 weeks.Hearing was tested by ABR.Serum homocysteine,folic acid were detected.Immune imprint method was used to analyze the expression of related protein.Cochlear tissue was tested by histological method to observe morphological changes.At the same time using the TUNEL method was used to analyse the cochlear tissue cell apoptosis.RESULTS Analysis showed that serum folate levels in mice fed without folic acid diet dropped than that of the normal group,while homocysteine levels elevated.Through the brain stem auditory records and cochlear cell apoptosis of mice severe hearing loss was found in folate deficiency group.Western blot detection showed enzyme catalysis homocysteine products increased by 50％ in folate deficiency group than that of the normal diet group.CONCLUSION The high homocysteine levels,and folate deficiency can cause hearing loss in mice and are related with the inner ear homocysteine metabolic disorders.关键词
叶酸缺乏/小鼠/听力障碍/动物实验Key words
Folic Acid Deficiency/Mice/Hearing Disorders/Animal Experimentation引用本文复制引用
蔡润茁,马云胜,左中夫..叶酸缺乏导致小鼠听力障碍机制的实验研究[J].中国耳鼻咽喉头颈外科,2017,24(12):620-624,5.基金项目
辽宁省自然科学基金(20170540394)和锦州医科大学奥鸿博泽基金(173914612)联合资助 （20170540394）
