中国耳鼻咽喉头颈外科2017，Vol.24Issue(12)：625-628,4.DOI:10.16066/j.1672-7002.2017.12.006
miR-34a介导Bcl-2促原代听觉皮层神经元凋亡的作用
MiR-34a induces Bcl-2 to promote the apoptosis of primary auditory cortex neuron
摘要
Abstract
OBJECTIVE To explore the role of miR-34a on upregulating the expression of Bcl-2,which induces the apoptosis of primary auditory cortex neuron in the central mechanism of age-related hearing loss.METHODS Using C57BL/6 suckling mouse to obtain the brain tissue from auditory cortex according to the Location map,and to primary culture the neurons.After transfection on primary neurons,western blot and immunofluorescence were used to detect the expression of Bcl-2 and then hoechst staining was used to detect the apoptosis after transfection.RESULTS primary culture of auditory cortex neurons abtained from enzyme digestion were transfected with miR-34a mimic and miR-34a inhibitor to upregualte or downregulate the expression of miR-34a,the results showed that the expression of Bcl-2 was decreased after upregulation of miR-34a with the concentration of 5 nmol/L(t=5.127,P＜0.05),there was significant difference between the concentration of 10 and 20 nmol/L(t=6.379,P＜0.05),while increased after downregulation of miR-34a with concentration of 5 nmol/L(t=4.926,P＜0.05),there was significant difference between the concentration of 10 and 20 nmol/L(t=5.821,P＜0.05).Hoechst staining showed that the apoptotic neurons was increased after transfection of miR-34a mimic.CONCLUSION miR-34a induce the auditory cortex neuron apoptosis through downregulates Bcl-2,which central mechanism of age-related hearing loss.关键词
动物实验/老年性聋/听觉皮质/神经元/细胞凋亡/B细胞淋巴瘤-2蛋白Key words
Animal Experimentation/Presbycusis/Auditory Cortex/Neurons/Apoptosis/B cell lymphoma-2引用本文复制引用
黄秋红,叶咏怡,郑亿庆..miR-34a介导Bcl-2促原代听觉皮层神经元凋亡的作用[J].中国耳鼻咽喉头颈外科,2017,24(12):625-628,4.基金项目
国家自然科学基金青年基金资助项目(81400456) （81400456）
