广西植物2018，Vol.38Issue(5)：545-551,7.DOI:10.11931/guihaia.gxzw201708030
HSCCC分离纯化未成熟罗汉果皂苷类化合物
Separation and purification of mogrosides from unripe Siraitia grosvenorii by high-speed countercurrent chromatography
摘要
Abstract
We established a method for separating mogrosides from unripe Siraitia grosvenorii by High-speed countercur-rent chromatography(HSCCC). Crude mogrosides fraction was enriched by macroporous resin method,and then separated by HSCCC. The two-phase solvent system composed of chloroform-methanol-n-butanol-water(5 : 6 : 1 : 4, v/v/v/v)was used for separation. The upper phase was used as the stationary phase,while the lower phase as the mo-bile phase.The rotation speed was 860 r·min-1,the flow rate was 2.5 mL·min-1,and the detection wavelength was 203 nm. 11-O-mogroside Ⅱ, mogroside Ⅱ E, 11-O-mogroside Ⅲ and mogroside Ⅲ, four compounds were prepared in one step,with the purity of 95.5%,98.2%,80.1%and 97.6%,respectively.This method that has the advantages of high sam-ple recovery rate,low loss and avoiding sample inactivation,which successfully realized the rapid and effective separa-tion of mogrosides. Hence,HSCCC can improve separation efficiency and provide theoretical foundation for the separa-tion and purification of moremogrosides,complementing and optimizing the method for separating mogrosides.关键词
高速逆流色谱/未成熟罗汉果/罗汉果皂苷ⅡE/11-O-罗汉果皂苷Ⅱ/罗汉果皂苷Ⅲ/11-O-罗汉果皂苷ⅢKey words
high-speed countercurrent chromatography(HSCCC)/unripe Siraitia grosvenorii/mogroside Ⅱ E/11-O-mogroside Ⅱ/mogroside Ⅲ/11-O-mogroside Ⅲ分类
农业科技引用本文复制引用
吴佩娟,卢凤来,羊学荣,符毓夏,李典鹏..HSCCC分离纯化未成熟罗汉果皂苷类化合物[J].广西植物,2018,38(5):545-551,7.基金项目
国家自然科学基金(81460411) （81460411）
广西重点研发计划项目(桂科AB16380108) （桂科AB16380108）
广西壮族自治区八桂学者专项项目[Supported by the National Natural Science Foundation of China(81460411) （81460411）
Guangxi Key R&D Program(AB16380108) （AB16380108）
Special Pro-gram of Guangxi Zhuang Autonomous Region Bagui Scholars]. （）
