广西植物2018，Vol.38Issue(5)：626-634,9.DOI:10.11931/guihaia.gxzw201705037
橙花瑞香的繁殖特性研究
Reproductive characteristics of Daphne aurantiaca
摘要
Abstract
Daphne has important medicinal and ornamental values and it is rich in resources in China. Low fruit setting rate is a feature of the genus,and limits its further development and utilization.As the machanism for low fruit setting is unclear and few related studies have been carried out,especially in China,almost no one has carried out such relevant work. We explored the reasons for low natural fruit setting rate of D.aurantiaca,using D.aurantiaca as research materi-al,through studying its sexual reproduction and pollination characteristics, including the measurement of floral traits, the detection of pollen viability by MTT solution and stigma receptivity by benzidine-H2O2solution,observation of stigma and pollen morphology by scanning electron microscope(SEM),and survey the visit rates of pollinators. The breeding system was tested by pollen ovule rate(P/O)and artificial pollination experiment.The results showed that pollen grains of D.aurantiaca had activities and stigmas had receptivities,and stigma and pollen developed normally through the SEM observation,which indicated that both male and female functions were normal.The flower structure of D.aurantiaca was special,flowers were very small,tubular structure,stigma-anther separation,with anthers consisting of two wheels ar-ranged in the middle and top of corolla tube while stigma in the base of corolla tube.During the day time we did not see any effective visitors,and at night we found the main pollinator of D. aurantiaca was moths,with low visiting rate. We only found two moths in the whole period of the visitor observation,and one moth visited three flowers and the other visi-ted two flowers in the same plant. Both P/O and artificial pollination experiments showed that the breeding system of D. aurantiaca was obligated out-crossing and self-incompatible.The fruit setting rate of D.aurantiaca was very low,only 1.4% in natural pollination and 23.3% in artificial cross-pollination. Its low fruit setting rate may be restricted by the large amount of flowers per plant,the inbreeding depression,the shortage of outcross pollen grains,resource limit and the influence of the flower structure. This study can provide theoretical basis for the utilization,preservation and expan-sion of D. aurantiaca resources.关键词
橙花瑞香/繁殖/传粉生物学/扫描电镜/坐果率Key words
Daphne aurantiaca/reproductive/pollination/scanning electron microscope/fruit setting rate分类
农业科技引用本文复制引用
刘淑娟,杨爱红,周华,余发新..橙花瑞香的繁殖特性研究[J].广西植物,2018,38(5):626-634,9.基金项目
江西省科技支撑计划项目(20151BBF60078) （20151BBF60078）
江西省科学院博士项目(2014-YYB-01) （2014-YYB-01）
江西省科学院普惠项目(2014-XTPH1-01) （2014-XTPH1-01）
南昌市优势科技创新团队项目(2015CXTD005)[Supported by Science and Technology Support Program of Jiangxi(20151BBF60078) （2015CXTD005）
Doctoral Program from Jiangxi Academy of Sciences(2014-YYB-01) （2014-YYB-01）
Pratt&Whitney Program from Jiangxi Aca-demy of Sciences(2014-XTPH1-01) （2014-XTPH1-01）
Nanchang Advantages of Science and Technology Innovation Team(2015CXTD005)]. （2015CXTD005）
