基础医学与临床2018，Vol.38Issue(6)：771-775,5.
Twist促进人三阴性乳腺癌细胞迁移和侵袭
Twist promotes cell migration and invasion in human breast cancer cell line Hs578T
摘要
Abstract
Objective To study the effects of Twist gene silencing on migration and invasion of human triple-nega-tive breast cancer cell Hs578T and its potential mechanisms. Methods The lentiviral vectors with Twist gene-targe-ted specific shRNA and the negative control were constructed,then transfected into the human triple-negative breast cancer cell line Hs578T to establish the stable cell lines of Twist gene silencing. The blank group(blank),negative control group(shNC) and Twist gene silencing group(shTwist) were set up.After screening by puromycin,the fluo-rescence expression and the infection efficiency of each group cells were observed by inverted fluorescence micro-scope. The expression of Twist mRNA and protein level was detected by quantitative real-time PCR and Western blot.Transwell migration and invasion experiments were performed to measure cell migration and invasion abilities, re-spectively. Western blot was used to detect the levels of p-AKT, AKT, p-ERK1/2 and ERK1/2 proteins. Results Twist gene was successfully knockdown in human triple-negative breast cancer cell line Hs578T. Compared with the blank group and shNC group cells,the cell migration and invasion ability of the shTwist group cells were decreased significantly(P<0.05,P<0.05),and the expression of Twist downstream p-AKT and p-ERK1/2 proteins were al-so reduced notably in the shTwist group cells(P<0.05,P<0.05). Conclusions Twist promotes cell migration and invasion via AKT/ERK signaling axis in human triple-negative breast cancer cell line Hs578T.关键词
Twist/乳腺癌/细胞迁移/细胞侵袭Key words
Twist/breast cancer/cell migration/cell invasion分类
医药卫生引用本文复制引用
张达容,王维莲,吴立翔,柳满然..Twist促进人三阴性乳腺癌细胞迁移和侵袭[J].基础医学与临床,2018,38(6):771-775,5.基金项目
国家自然科学基金(31171336) （31171336）
