基础医学与临床2018，Vol.38Issue(6)：809-814,6.
抑制BCAR1降低肺癌细胞系A549 p-P38表达
Inhibition of BCAR1 decreases p-P38 expression in lung cancer cell line A549
摘要
Abstract
Objective To discuss the effect of breast cancer anti-estrogen resistance 1(BCAR1) knockdown on the expression of P38 and p-P38 in lung cancer cell line A549. Methods A549 cells (control group), A549 cells with RNAi letiviral vector of BCAR1 (interference group) and A549 cells with negative control letiviral vector (negative group) were cultured. Western blot was used to detect the expression of P38 and p-P38. The prolifera-tion,cell cycle,migration and invasion were measured by colony formation assay,flow cytometer,transwell experi-ment and scratch adhesion test,respectively. Results p-P38 expression in interference group cells was significant-ly lower than that in other two group cells(P<0.05).G1phase of interference group cells was significantly increas-ing than that in other two group cells(P<0.05).The proliferation,migration and invasion of interference group cells were all significantly suppressed as compared with that of other two group cells(P<0.05). Conclusions BCAR1 knockdown decreases p-P38 expression and inhibits proliferation,migration and invasion of A549 cells.关键词
BCAR1/p-P38/P38/A549细胞/肺癌Key words
BCAR1/p-P38/P38/A549 cells/lung cancer分类
医药卫生引用本文复制引用
黄伟,阮姝琴,王如文,范小青..抑制BCAR1降低肺癌细胞系A549 p-P38表达[J].基础医学与临床,2018,38(6):809-814,6.基金项目
重庆市渝中区科技计划项目(20150128) （20150128）
