极地研究2018，Vol.30Issue(2)：192-198,7.DOI:10.13679/j.jdyj.20170010
基于DNA条形码对南极Yelcho站周边海域鱼类的种类鉴定
IDENTIFICATION OF ANTARCTIC FISH FROM THE SEAADJACENT TO YELCHO STATION BASED ON DNA BARCODING
摘要
Abstract
As an important part of the Antarctic Ocean ecosystem, Antarctic fish play a role in material cycling and the energy flow within the food web. Individual Antarctic fish specimens were collected from the sea adjacent to Yelcho Station during the 32nd Chinese National Antarctic Research Expedition, and morpho-logical analysis and DNA barcoding were employed in their identification. After running blast searches in NCBI, sequence similarity results showed that only two individuals were identifiable to the species level, while six individuals were unmatched. Combined with a homologous sequence from GenBank, phylogenetic analysis was performed and four groups were unambiguously identified from the NJ tree. There was clear overlap (barcoding gap) between intraspecific and interspecific variabilities in each species, as the smallest interspecific divergences were well below 2％, while the largest intraspecific divergences were above 2％. Three valid species were detected from eight individuals, encompassing seven Nototheniidae fish and one Harpagiferidae fish. Finally, all results further confirmed that notothenioid fish were dominant in terms of species diversity and biomass in the investigated seas.关键词
南极鱼科/DNA条形码/序列相似性/条形码间隙/遗传距离Key words
Nototheniidae/DNA barcoding/sequence similarity/barcoding gap/genetic distance引用本文复制引用
李渊,张然,宋普庆,李海,陈坚,林龙山..基于DNA条形码对南极Yelcho站周边海域鱼类的种类鉴定[J].极地研究,2018,30(2):192-198,7.基金项目
南北极环境综合考察与评估专项(CHINARE 2016-01-05 （）
CHINARE 2016-03-05)资助 （）
