解放军医学杂志2017，Vol.42Issue(9)：769-774,6.DOI:10.11855/j.issn.0577-7402.2017.09-04
新生小鼠心肌肌钙蛋白I相互作用蛋白的鉴定与功能预测
Identification and function prediction of proteins interacted with cTnI in mouse cardiac myocytes
摘要
Abstract
Objective To isolate and identify the proteins that interact with cardiac troponin I (cTnI) in primary mouse cardiomyocytes,and to analyze their biological processes and signaling pathways by use of High throughput proteomics and bioinformatics techniques.Methods The hearts of C57 neonatal mice were used to prepare the primary cardiomyocytes.The cTnI binding proteins were extracted from the whole cell protein by co-immunoprecipitation,and the proteins were analyzed and identified by mass spectrometry.Furthermore,the physicochemical properties,biological processes and signaling pathways of cTnI interacting proteins were predicted by bioinformatics tools.Results A total of 262 proteins were identified by mass spectrometry,cTnI binding proteins were involved in 14 biological processes and 33 KEGG biological pathways (P＜0.05).Conclusion In mouse cardiomyocytes,262 kinds of proteins may interact with cTnI,and participate in regulation of calcium ion influx and PI3K-Akt signaling pathways.关键词
心肌肌钙蛋白I/蛋白质组学/生物信息学/PI3K-Akt信号通路Key words
cardiac troponin I/proteomics/bioinformatics/PI3K-Akt signaling pathway分类
医药卫生引用本文复制引用
王致远,赵唯安,刘玲娟,刘振国,田杰..新生小鼠心肌肌钙蛋白I相互作用蛋白的鉴定与功能预测[J].解放军医学杂志,2017,42(9):769-774,6.基金项目
国家自然科学基金面上项目(81670212)This work was supported by the National Natural Science Foundation of China (81670212) （81670212）
