解放军医学杂志2017，Vol.42Issue(9)：781-787,7.DOI:10.11855/j.issn.0577-7402.2017.09.06
PUMA-α蛋白在高糖诱导的人腹膜间皮细胞凋亡及纤维化中的作用
Effects of protein PUMA-α on the apoptosis and fibrosis of human peritoneal mesothelial cells induced by high glucose
车明文 1李凤容 1龚莉芳 1方秀凤 1邓海平 2蒋希 3唐亚为 4王汉民5
作者信息
- 1. 841000 新疆库尔勒 解放军273医院内三科
- 2. 831100 新疆昌吉 解放军69008部队卫生队
- 3. 841000 新疆库尔勒 解放军273医院内一科
- 4. 710032 西安 陕西省友谊医院消化内科
- 5. 710032 西安 第四军医大学西京医院肾脏内科
- 折叠
摘要
Abstract
Objective To explore the effects of p53 up-regulated modulator of apoptosis (PUMA)-α protein on the apoptosis and fibrosis of human peritoneal mesothelial cells (HPMCs) induced by high glucose.Methods HPMCs were induced by 50mmol/L D type glucose or mannitol for 72 hours respectively,flow cytometry was employed to detect the rate of apoptotic cells,and theexpression levels of apoptosis-and fibrosis-related proteins were detected by Western blotting.The untreated HPMCs were transfected with Lenti-PUMA-α,and the treated cells were transfected with shRNA-PUMA-α,the number of apoptotic cells and the expression levels of apoptosis-and fibrosis-related proteins were detected with the methods mentioned above.Results Flow cytometry showed that the rate of apoptotic HPMCs increased after being induced by high glucose for 72 hours,and Western blotting revealed that the expression levels of pro-apoptotic and pro-fibrotic related proteins increased,but the arrestins of apoptosis and fibrosis-related proteins decreased.Up-regulation of PUMA-α promoted apoptosis and fibrosis,while down-regulation of PUMA-α alleviated apoptosis and fibrosis of HPMCs.Conclusion High glucose may accelerate apoptosis and fibrosis of HPMCs by up-regulating the expression of PUMA-α.关键词
细胞凋亡/PUMA-α蛋白/人腹膜间皮细胞Key words
apoptosis/PUMA-α protein/human peritoneal mesothelial cells分类
医药卫生引用本文复制引用
车明文,李凤容,龚莉芳,方秀凤,邓海平,蒋希,唐亚为,王汉民..PUMA-α蛋白在高糖诱导的人腹膜间皮细胞凋亡及纤维化中的作用[J].解放军医学杂志,2017,42(9):781-787,7.
