解放军医学杂志2017，Vol.42Issue(9)：788-792,5.DOI:10.11855/j.issn.0577-7402.2017.09.07
自体与异体脂肪间充质干细胞移植治疗大鼠急性心肌梗死的比较研究
A comparative study on autologous and allogeneic adipose derived mesenchymal stem cell transplantation in treatment of acute myocardial infarction rats
汪兆艳 1郭建巍 2杨印祥 3栾佐2
作者信息
- 1. 100850 北京 军事医学科学院基础医学研究所
- 2. 100048 北京 海军总医院儿科
- 3. 100048 北京 海军总医院检验科
- 折叠
摘要
Abstract
Objective To investigate the effects of autologous and allogeneic adipose derived mesenchymal stem cells (ADMSCs) transplantation on rat model of acute myocardial infarction (AMI) and possible mechanisms.Methods The AMI models were established with 45 male Lewis rats by ligation of left anterior descending coronary artery,and then randomly divided into 3 groups (15 each) including AMI group,allogeneic ADMSC transplantation group (Allo-ADMSC group) and autologous ADMSC transplantation group (Auto-ADMSC group).After successfully modeling,CM-Dil-labeled third-generation ADMSCs (2 × 106) were implanted into the myocardium of rats within 1 hour,and rats in AMI group were injected with equal amount of PBS.The cardiac function,immunofluorescence and Masson were identified 4 weeks after transplantation.Results Four weeks after transplantation,compared with AMI group,the left ventricular ejection fraction in Allo-ADMSC group and Auto-ADMSC group increased significantly,the left ventricular end-systolic and end-diastolic diameter decreased,the collagen deposition fraction decreased significantly (P＜0.05).Compared with Allo-ADMSC group,the left ventricular ejection fraction increased in AutoADMSC group,the number of newborn capillaries increased and the myocardial collagen deposition fraction decreased (P＜0.05).Conclusion Autologous ADMSC can promote vascular proliferation in the infarct area more better than allogeneic ADMSC,reduce the local collagen deposition,extenuate the degree of myocardial fibrosis,thereby to inhibit collagen remodeling,and repair damaged myocardial tissue and improve heart function.关键词
心肌梗死/脂肪间充质干细胞/移植Key words
myocardial infarction/adipose tissue-derived stem cells/transplantation分类
医药卫生引用本文复制引用
汪兆艳,郭建巍,杨印祥,栾佐..自体与异体脂肪间充质干细胞移植治疗大鼠急性心肌梗死的比较研究[J].解放军医学杂志,2017,42(9):788-792,5.
