解放军医学杂志2017，Vol.42Issue(9)：793-798,6.DOI:10.11855/j.issn.0577-7402.2017.09.08
结肠组织中Th1/Th2和Th17细胞失衡与溃疡性结肠炎的关系
Association of ulcerative colitis with the imbalance between Th1, Th2 and Th17 cells in the colonic tissues
摘要
Abstract
Objects To explore the association of ulcerative colitis (UC) with the imbalance between Th1,Th 2 and Th17 cells in the colonic tissues.Methods A total of 41 UC patients and 52 controls was recruited in the present study.The real-time fluorescent quantitative PCR was applied for detecting the mRNA levels of Thl,Th2 and Th 17 cells-associated transcription factors T-bet,GATA-3 and RORγt and cytokines IFN-γ,IL-4 and IL-17A in the colonic tissues.Simultaneously,the expressions of IFN-γ,IL-4 and IL-17A in the colonic tissues were also examined by an immunohistochemical staining method.Results Compared with the controls,the mRNA expressions of GATA-3,RORγt and IL-17A were more significantly enhanced in UC patients (0.84 ± 0.24 vs.0.69 ± 0.22,P=0.002;0.99 ± 0.29 vs.0.83 ± 0.23,P=0.004;1.59 ± 0.65 vs.1.35 ± 0.43,P=0.035).According to the ＂Truelove and Witts Severity Index＂,those patients were divided into different subgroups.The mRNA expressions of GATA-3,RORγt,and IL-17A were shown to be higher in patients with moderate and severe UC than in those with mild UC (0.90 ± 0.18 vs.0.78 ± 0.16,P=0.030;1.11 ± 0.31 vs.0.87 ± 0.26,P=0.011;1.83 ± 0.64 vs.1.34 ± 0.66,P=0.020).Moreover,the immunohistochemistry results demonstrated that the IL-17A positive cells were positioned mainly in the intestinal epithelial layer and lamina propria.Compared to the controls,the mean integral optic density of IL-17A was significantly increased in the colonic tissues of UC patients (0.25 ± 0.07 vs.0.13 ± 0.03,P＜0.001).The similar results were obtained for IL-17A in patients with moderate and severe UC when compared to those with mild UC (0.31 ± 0.07 vs.0.19 ± 0.06,P＜0.001).In contrast to the controls,the mRNA ratios ofGATA-3/T-bet,RORγt/ T-bet and RORγt/GATA-3 were significantly higher in the tissues of colonic UC patients (1.12 ± 0.30 vs.0.96 ± 0.31,P=0.014;1.33 ± 0.37 vs.1.15 ± 0.33,P=0.015;1.44 ± 0.45 vs.1.20 ± 0.42,P=0.009),and in the patients,the mRNA ratios for GATA-3/T-bet,RORγt/T-bet and RORγt/GATA-3 were significantly higher in the patients with moderate and severe UC than in those with mild UC (1.27 ± 0.35 vs.1.00 ± 0.32,P＜0.001;1.45 ± 0.37 vs.1.19 ± 0.36,P=0.028;1.59 ± 0.43 vs.1.28 ± 0.46,P=0.031).Conclusions These findings suggest that the imbalance between Thl,Th2 and Th17 cells in the colonic tissues may be implicated in UC.关键词
Th1/Th2细胞/Th17细胞/溃疡性结肠炎Key words
Th1/Th2 cells/Th17 cells/ulcerative colitis分类
医药卫生引用本文复制引用
吴昊,夏盛隆,夏宣平,徐昌隆,胡定元,邵晓晓,蒋益..结肠组织中Th1/Th2和Th17细胞失衡与溃疡性结肠炎的关系[J].解放军医学杂志,2017,42(9):793-798,6.基金项目
浙江省自然科学基金(LY14H030012,LY15H030018,LY16H 160055,LY17H030011) （LY14H030012,LY15H030018,LY16H 160055,LY17H030011）
浙江省卫生科技计划资助项目(2012KYA132) （2012KYA132）
温州市科技局资助项目(Y20150157,Y20160102)This work was supported by the Natural Science Fundation of Zhejiang Province (LY14H030012,LY15H030018,LY16H160055,LY17H030011) （Y20150157,Y20160102）
Health Department of Zhejiang (2012KYA132) （2012KYA132）
and the Science and Technology Bureau of Wenzhou (Y20150157,Y20160102) （Y20150157,Y20160102）
