江苏大学学报（医学版）2018，Vol.28Issue(3)：190-194,5.DOI:10.13312/j.issn.1671-7783.y180051
丹参酮ⅡA磺酸钠抑制低氧诱导的大鼠肺动脉平滑肌细胞增殖及其机制
Sodium tanshinone ⅡA sulfonate inhibits the proliferation of hypoxia-induced PASMC of rat and its potential mechanism
摘要
Abstract
Objective:To investigate the effect of sodium tanshinoneⅡA sulfonate(STS) on the pro-liferation of rat pulmonary arterial smooth muscle cells(PASMC) induced by hypoxia and its potential mechanism. Methods:Rat PASMC were selected for the following experiments. First,PASMC were di-vided into normoxia control group,hypoxia group(3% O2),normoxia control +STS low-, medium- and high-dose groups(5,10,20 ng/mL). After cultured for 60 h, the proliferation of PASMC was deter-mined by CCK-8. Then,PASMC were divided into normoxia control group,hypoxia group(3% O2),hy-poxia +STS group(10 ng/mL),qRT-PCR was used to detect the mRNA expressions of mTOR,eIF2α, c-myc. In mechanism study test,PASMC were divided into normoxia control group, hypoxia group(3% O2),hypoxia +STS group(10 ng/mL), hypoxia +rapamycin group(20 nmol/L); Western blotting were used to investigate the expressions of mTOR, p-mTOR, eIF2α, p-eIF2α, c-myc in PASMC. Re-sults:Compared with normoxia control group,cell viability in hypoxia group increased significantly(P<0.05); compared with hypoxia group, cell viability in STS group decreased significantly(P <0.05). qRT-PCR showed the mRNA expression of mTOR,eIF2α and c-myc in hypoxia group increased signifi-cantly in contrast to normoxia control group(P<0.05);compared with hypoxia group,the three mRNAs expression in STS group decreased significantly(P<0.05). In Western blotting,compared with normox-ia control group,the expression of mTOR, p-mTOR, eIF2α, p-eIF2α, c-myc increased significantly in hypoxia group(P<0.05); while in contrast to hypoxia group, the expression of mTOR, p-mTOR, eIF2α, p-eIF2α,c-myc decreased significantly(P<0.05);there was no statistical significance between hypoxia+STS group and hypoxia+rapamycin group(P>0.05). Conclusion:STS could inhibit hypoxi-a-induced proliferation of PASMC by suppressing mTOR/eIF2α signaling pathway.关键词
大鼠肺动脉平滑肌细胞/丹参酮ⅡA磺酸钠/mTOR/eIF2α/c-mycKey words
rat pulmonary arterial smooth muscle cells/sodium tanshinone ⅡA sulfonate/mTOR/eIF2α/c-myc分类
医药卫生引用本文复制引用
杨磊,郑金旭,史小东,钱海,孙金玲..丹参酮ⅡA磺酸钠抑制低氧诱导的大鼠肺动脉平滑肌细胞增殖及其机制[J].江苏大学学报（医学版）,2018,28(3):190-194,5.基金项目
上海市自然科学基金资助项目(10ZR1422600) （10ZR1422600）
江苏大学横向合作项目(1803240001) （1803240001）
