军事医学2017，Vol.41Issue(11)：894-897,4.DOI:10.7644/j.issn.1674-9960.2017.11.006
睡眠剥夺对大鼠脾脏淋巴细胞生物钟基因表达水平的影响
Effects of sleep deprivation on spleen circadian clock gene expression in rats
摘要
Abstract
Objective To investigate the effects of 72 h sleep deprivation (SD) on circadian clock gene expression in the rat spleen.Methods The rats were randomly divided into control group and SD group.An SD instrument was used to deprive the rats of sleep for 72 h.Then the lymphocytes from the spleen were obtained by Ficoll seperation medium before the expression of clock genes was detected by RT-PCR and Western blotting analysis respectively.Results Compared with the control group,the mRNA levels of bmal1,clock,per2 and rev-erbα strikingly decreased in the spleens of the SD group rats.However,npas2,per1,rorα and cry1 mRNA expression hardly changed in the control and SD group.Meanwhile,the protein levels of spleen BMAL1,NPAS2,CRY1 and RORα were significantly down-regulated and PER1 protein levels were up-regulated in SD group compared with control group.However REV-ERBα protein expression remained unchanged in the control and SD group.Conclusion 72 h SD can result in abnormal expressions of several circadian clock genes in lymphocytes of the spleen at both transcription and translation levels.关键词
睡眠剥夺/昼夜节律/生物钟/基因表达/淋巴细胞Key words
sleep deprivation/circadian rhythm/circadian clock/gene expression/lymphocytes分类
医药卫生引用本文复制引用
邢陈,顾晔,徐秀段,邹书仙,胡永亮,胡美茹,宋伦..睡眠剥夺对大鼠脾脏淋巴细胞生物钟基因表达水平的影响[J].军事医学,2017,41(11):894-897,4.基金项目
北京市自然科学基金青年资助项目(7164287) （7164287）
全军医学科研“十二五”重大项目(AWS14J011) （AWS14J011）
