军事医学2017，Vol.41Issue(11)：906-911,6.DOI:10.7644/j.issn.1674-9960.2017.11.009
基于面阵CCD的便携式上转换发光免疫层析检测系统
An area CCD-based detection system for up-converting phosphor immune lateral flow assay
摘要
Abstract
Objective To make portable the detector for up-converting phosphor (UCP) immune lateral flow assay portable and increase the test precision by developing an area CCD-based detection system for UCP immune lateral flow assay.Methods The excitation light source was a 980 nm and 300 mW laser diode.In order to decrease the error induced by the un-uniformity of laser irradiation,a uniformity mirror was inserted in the beam and a calibration algorithm was optimized.The residual error from the un-uniformity irradiation was less than 1％.Results Thanks to the adjustability of the exposure time,the dynamic range of detection of the system was as high as 106 dB.The repeat test error for the very low signal was 1％ (variation coefficient).The linear correlation coefficient between the tested T/C value and sample concentration was 0.998.Conclusion Compared to is traditional instrument,this detection system is static,portable and highly precise.关键词
面阵CCD/上转换发光/免疫层析/生物传感技术/激光匀光Key words
area CCD/up-converting phosphor/immune chromatography/biosensing techniques/laser uniformity分类
机械制造引用本文复制引用
李抄,杨子健,杜耀华,吴太虎,陈锋..基于面阵CCD的便携式上转换发光免疫层析检测系统[J].军事医学,2017,41(11):906-911,6.基金项目
国家重点研发计划资助项目(2016YFC1201404) （2016YFC1201404）
军队重点课题资助项目(BWS16J009,AWS13C007) （BWS16J009,AWS13C007）
