农业环境科学学报2018，Vol.37Issue(5)：926-932,7.DOI:10.11654/jaes.2017-0954
一株土著B[a]P降解菌的筛选及降解特性研究
The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria
摘要
Abstract
Eight bacteria strains were isolated from the soil contaminated with petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs)using repeated subcultures in liquid medium with added benzo[a]pyrene(B[a]P)as the sole carbon source. One of the bacterial strains named BB-1 had an optimal degradation effect(100% homologous to Bacillus megaterium)through the 16sRNA gene sequencing method. In order to explore the degradation character of BB-1,the strain was cultured under pH=7.0,30 °C for 8 days, after which the B[a]P degradation rate reached a maximum of 66.36%.In order to investigate the degradation rate of BB-1 under varying conditions of initial pH and additional sucrose concentration in the culture medium,the incubation was carried out at 30°C for 8 days,with various values of initial pH including 4.0, 6.0, 8.0, and 10.0. The results showed that the degradation rates of B [a]P were 12.14%,39.61%,55.21%, and 30.03%,respectively.In other words,the highest degradation efficiency was achieved for pH 8.0.With the addition of 0.1% and 0.5% sucrose as an additional carbon source, the degradation rate of B[a]P reached 70.56% and 74.89%, respectively. It was thus clear that the highest degradation efficiency occurred in the media with the addition of 0.5% sucrose.关键词
PAHs/B[a]P/污染土壤/生物降解Key words
polycyclic aromatic hydrocarbons(PAHs)/benzo[a]pyrene(B[a]P)/contaminated soil/biodegradation分类
农业科技引用本文复制引用
顾平,周启星,王鑫,伍跃辉,陈威,张倩茹,龚起,周爱申..一株土著B[a]P降解菌的筛选及降解特性研究[J].农业环境科学学报,2018,37(5):926-932,7.基金项目
中央高校基本科研业务费专项资金项目.A Special Fund for Basic Scientific Research Services of Central Colleges and Universities （）
