农业环境科学学报2018，Vol.37Issue(5)：984-991,8.DOI:10.11654/jaes.2017-1246
洪泽湖湿地不同植物作用下沉积物细菌群落结构
Bacterial community structures in sediments are affected by different plants in Hongze Lake wetlands
摘要
Abstract
To study the effects of plant species on sediment bacterial community structure, we collected samples from three different wet-lands dominated by Nelumbo nucifera,Zizania caduciflora,and Phragmites australis in the Hongze Lake area and analyzed the samples us-ing the high-throughput sequencing technique.The results showed that the number of Operational Taxonomic Units(OTUs)for each sample from the three wetlands were 1494,1503,and 1600,respectively.At the phylum level,the dominant phyla in the sediments were Proteobac-teria, Chloroflexi,Acidobacteria,Nitrospirae, Bacteroidetes, and Firmicutes. At the genus level, high abundances of Nitrospira and Nitro-somonadaceae,which are mainly involved in nitrification,were observed in all sediment samples.The results also showed that the bacterial community structure at the Phragmites australis site was similar to that at the Zizania caduciflora site. The TOC and TN contents at the Nelumbo nucifera site were significantly lower than at the Phragmites australis and Zizania caduciflora sites.Furthermore,significant differ-ences in bacterial community structure were observed between the Nelumbo nucifera site and the Phragmites australis or Zizania caduciflora sites.The relative abundances of Methylotenera,Bacillus,Pseudomonas,and Lactococcus,with their high nitrification capacities,meant that nitrification at the Nelumbo nucifera site was over 10 times higher than at the Phragmites australis or Zizania caduciflora sites.This indicat-ed that the plant species had a significant influence on the C/N contents and bacterial community structure in the wetland sediments.Fur-thermore,Nelumbo nucifera has a greater potential for denitrification compared to Zizania caduciflora and Phragmites australis,which means that this plant can reduce the risk of eutrophication.关键词
高通量测序/细菌群落/沉积物/洪泽湖Key words
high-throughput sequencing/bacterial community/sediment/Hongze Lake分类
资源环境引用本文复制引用
伍贤军,杨红,程睿,盛怡,韩建刚,李萍萍..洪泽湖湿地不同植物作用下沉积物细菌群落结构[J].农业环境科学学报,2018,37(5):984-991,8.基金项目
国家自然科学基金项目(41375149,0901112) （41375149,0901112）
江苏省博士后科研资助计划项目(1501020A) （1501020A）
江苏省高校优势学科建设工程项目(PAPD).The National Natural Science Foundation of China(41375149,0901112) （PAPD）
Jiangsu Planned Projects for Postdoctoral Research Funds(1501020A) （1501020A）
Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD) （PAPD）
