四川医学2018，Vol.39Issue(3)：270-272,3.DOI:10.16252/j.cnki.issn1004-0501-2018.03.006
四氯化碳不同注射方式诱导大鼠不同分期肝纤维化模型的研究
Study on Different Stages of Hepatic Fibrosis Induced by Different Injection of Carbon Tetrachloride in Rats
摘要
Abstract
Objective To study the effect of different injection methods of carbon tetrachloride on the establishment of different stages of hepatic fibrosis models in rats,and to provide a reliable method for carbon tetrachloride induced different stages of hepatic fibrosis models in rats.Methods 42 male SD rats were randomly divided into subcutaneous and intraperitoneal injection group,carbon tetrachloride-edible blend oil suspension was injected 2 times a week in hind leg medial skin and abdominal cavity. From the sixth week,2-3 rats were executed in each group for hepatic fibrosis staging.Results During the modeling period,3 rats died in the subcutaneous injection group,the mortality rate was 14.3%;11 cases died in the intraperitoneal injection group,the mortality rate was 52.4%,and the mortality of the two injection methods was obviously different(P<0.05).Subcutaneous injec-tion group used seven weeks to get significant liver fibrosis,twelve weeks to finish modeling,and obtained 11 rats with significant hepatic fibrosis.Intraperitoneal injection group used six weeks to get significant liver fibrosis,ten weeks to finish modeling,and ob-tained 10 rats with significant hepatic fibrosis.There was no significant difference between the two injection methods obtaining sig-nificant hepatic fibrosis(P>0.05).Conclusion The liver fibrosis models of rats can be established by intraperitoneal and subcu-taneous injection methods;the mortality of subcutaneous injection molding was lower than that of intraperitoneal injection,and the model completion time was longer.There was no significant difference between two injection methods in obtaining significant hepat-ic fibrosis.关键词
四氯化碳/肝纤维化模型/大鼠Key words
carbon tetrachloride/hepatic fibrosis model/rats分类
医药卫生引用本文复制引用
金明丽,潘志华,舒健,卢欣,王晓凤,柴丽..四氯化碳不同注射方式诱导大鼠不同分期肝纤维化模型的研究[J].四川医学,2018,39(3):270-272,3.基金项目
四川省卫生和计划生育委员会科研课题(编号:17PJ436) （编号:17PJ436）
