山东医药2017，Vol.57Issue(8)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.08.003
血清HBcrAg、线性化HBsAg对隐匿性乙型肝炎的诊断价值
Diagnostic value of serum HBcrAg and linearized HBsAg for occult hepatitis B virus infection
摘要
Abstract
Objective To investigate the value of serum hepatitis B virus core-related antigens (HBcrAg) and linearized hepatitis B surface antigen (HBsAg) in diagnosis of occult hepatitis B virus infection (OBI) in Chinese population.Methods HBcrAg and linearized HBsAg levels were measured by chemiluminescence enzyme immunoassay (CLEIA) in 63 patients with OBI (OBI group) and 150 healthy people (control group).The serum HBV DNA was detected by fluorescence quantitative PCR.Receiver operating characteristics (ROC) was analyzed to calculate diagnostic accuracy of HBcrAg and combination with linearized HBsAg.Results Serum HBcrAg and linearized HBsAg were detected in 90.5％ and 42.9％ of the OBI group,and 10.7％,6.0％ of the control group,respectively (x2 =125.5,42.9,all P ＜ 0.05).There were positive correlation between HBV DNA and HBcrAg,HBV DNA and linearized HBsAg,HBcrAg and linearized HBsAg,respectively (R2 =0.471 4,0.122 2,0.086 8,P ＜ 0.05 or P ＜ 0.01).HBcrAg had better area under the ROC curves (AUC) [0.911 (95％ CI:0.864-0.946)] than linearized HBsAg [0.683 (95％ CI:0.616-0.745),Z=6.134,P ＜ 0.05].The AUC of combined detection was 0.895 (95％ CI:0.846-0.933),and no significant improvement was found (Z =0.657,P ＞ 0.05).The optimal HBcrAg and linearized HBsAg cutoff values were 100 U/mL and 0.48 IU/mL,respectively.The sensitivity of HBcrAg in diagnosis of OBI was significantly higher than that of linearized HBsAg (x2 =121.4,P ＜ 0.01),while specificity was a little lower (x2 =3.034,P ＞ 0.05).Combined detection in diagnosis of OBI did not show any improvement than single detection of HBcrAg or linearized HBsAg in sensitivity or specificity (x2 =1.322,3.802,all P ＞ 0.05).Conclusion There is a good correlation between serum HBcrAg and HBV DNA,and HBcrAg is more sensitive than linearized HBsAg or combined detection in diagnosis of OBI,which can be used as a good serological marker for the diagnosis of OBI.关键词
隐匿性乙型肝炎/乙肝病毒核心相关抗原/线性化乙型肝炎病毒表面抗原Key words
occult hepatitis B virus infection/Hepatitis B virus core-related antigens/linearized hepatitis B surface antigen分类
医药卫生引用本文复制引用
朱嫦琳,薛雄燕,陈展泽,谷愉愉,许扬扬,李炜煊..血清HBcrAg、线性化HBsAg对隐匿性乙型肝炎的诊断价值[J].山东医药,2017,57(8):9-12,4.基金项目
广东省医学科研基金立项项目(B2015127). （B2015127）
