山东医药2017，Vol.57Issue(8)：13-16,4.DOI:10.3969/j.issn.1002-266X.2017.08.004
多烯紫杉醇联合反义miR-21对人胰腺癌细胞增殖和凋亡的影响
Effect of docetaxel combined with antisense miR-21 on proliferation and apoptosis of human pancreatic cancer cell
摘要
Abstract
Objective To investigate the effect of docetaxel combined with antisense miR-21 on proliferation and apoptosis of human pancreatic cancer cell line MiaPaCa-2,and to discuss its possible mechanism.Methods Seven concentrations of docetaxel (DTX) were selected to detect their proliferation inhibition rates on human pancreatic cancer MiaPaCa2 cells to choose the best IC5o (15 μmol/L) which was then used to do the following experiment.Human pancreatic cancer MiaPaCa-2 cells were divided into the control group,antisense miR-21 group,DTX group,AS-miR-21 group,and DTX + AS-miR-21 group.Cells in the AS-miR-21 group and DTX + AS-miR-21 group were transfected by AS-miR-21,and the antisense miR-21 group was transfected by Lipofectamine2000 for 24 h.Cells in the DTX + AS-miR-21 group were treated with DTX,and DTX group was treated with 15 μmol/L DTX for 48 h.RT-PCR was used to detect the expression of miR21 in each group.The proliferation capacity of cells was detected by the cloning formation assay.The apoptosis rate was measured by flow cytometry.The expression of cyclindependent kinase 5 (CDK5) in each group was evaluated by Western blotting.Results The relative expression of miR-21 in the AS-miR-21 group and DTX + AS miR-21 group was significantly lower than that in the control group,and antisense miR-21 group,which indicated that the transfection was successful.Compared with the control group and antisense miR-21 group,the number of clones in the DTX group,AS-miR-21 group and DTX + AS-miR-21 group was significantly decreased,the early apoptosis rate was significantly increased,and the relative expression of CDK5 protein was significantly decreased,and the most significant changes were found in the DTX + AS-miR-21 group (all P ＜0.05).Conclusion DTX combined with AS-miR-21 can significantly inhibit the proliferation of human pancreatic cancer cells and induce the apoptosis,which may be associated with the decrease of CDK5 protein expression.关键词
胰腺癌/多烯紫杉醇/微小RNA-21/细胞增殖/细胞凋亡Key words
pancreatic carcinoma/docetaxol/miR-21/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
赵欣,王春立,李楠,马涛,尤胜义..多烯紫杉醇联合反义miR-21对人胰腺癌细胞增殖和凋亡的影响[J].山东医药,2017,57(8):13-16,4.基金项目
天津市科技计划项目(12ZCZDSY03500). （12ZCZDSY03500）
