山东医药2017，Vol.57Issue(36)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.36.002
沉默HMGB1基因对非小细胞肺癌细胞上皮间质转化的影响及作用机制
Effect of silencing HMGB1 on epithelial mesenchymal transition of non-small-cell lung cancer
摘要
Abstract
Objective To explore the effect of high mobility group box 1 (HMGB1) on epithelial mesenchymal transition (EMT) of non-small-cell lung cancer (NSCLC) and its mechanism.Methods NSCLC A549 cells in the third generation were randomly divided into the si-HMGB1 group,negative control group,and blank group.Cells in the si-HMGB1 group and negative control group were transfected with HMGB1 small interfering RNA (siRNA) sequence,HMGB1 negative control siRNA transfection,and cells in the blank group were not transfected.After 72 h,the HMGB1 silencing efficiency of each group was verified.The expression of EMT-related proteins (E-cadherin,V-cadherin,Vimentin) and signal pathway-related proteins (Snail,NF-κB p65,p-NF-κB p65) in cells collected after 72-hour transfection was detected by Western blotting.Results The expression of HMGB1 mRNA in the si-HMGB1 group was significantly lower than that in the negative control group and blank group (P ＜ 0.05),and there was no significant difference between the negative control group and the blank group (P ＞ 0.05).The protein expression of E-cadherin and VDR of the si-HMGB1 group was significantly higher,and the protein expression of N-cadherin and Vimentin was significantly lower than that of the negative control group and blank group (all P ＜ 0.05),and there was no significant difference between the negative control group and the blank group (P ＞ 0.05).The expression of Snail,p-NF-κB p65 protein of the si-HMGB1 group was lower than that of the negative control group and the blank group (P ＜ 0.05),and there was no significant difference between the negative control group and the blank group (P ＞ 0.05).No significant difference was found in the expression of p-NF-κB p65 among these three groups (all P ＞ 0.05).Conclusion HMGB1 silencing can reverse the EMT process in A549 cells by blocking the Snail/NF-κB signaling pathway.关键词
非小细胞肺癌/高迁移率族蛋白1/上皮间质转化/基因沉默Key words
non-small-cell lung cancer/high mobility group box 1/epithelial mesenchymal transition/gene silencing分类
医药卫生引用本文复制引用
蒲江涛,唐小军,宋琦,胡智,何开明,吴云飞,戴天阳..沉默HMGB1基因对非小细胞肺癌细胞上皮间质转化的影响及作用机制[J].山东医药,2017,57(36):5-8,4.基金项目
卫生部医药卫生科技发展研究(卫技中[2011]24号). （卫技中[2011]24号）
