山东医药2017，Vol.57Issue(44)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.44.001
力达霉素对人宫颈癌Caski细胞增殖的影响及其机制
Effect of lidamycin on proliferation of human cervical cancer Caski cells
摘要
Abstract
Objective To investigate the effect of lidamycin on the proliferation of human cervical cancer Caski cells and its underlying molecular mechanisms.Methods Human cervical cancer Caski cells were randomly divided the experimental groups (including the low-dose,medium-dose and high-dose groups) which were separately treated with 1,2.5 and 5 ng/mL lidamycin (100 μL) for 24 h,and the control group which was added with the same volume of RPMI-1640.MTT assay was used to detect the inhibition rate of proliferation.The cell cycle and mitochondrial membrane potential of CasKi cells were measured by flow cytometry.Transmission electronic microscope was used to observe the morphological changes of Caski cells.We calculated the apoptosis rate and mitotic catastrophe rate.Results The proliferation rate and survival rate of the experimental groups were significantly lower than those of the control group,and the decrease in the high-dose group was more significant (all P ＜ 0.05).The percentage of cells in the S phase of the control group and the medium-dose group was higher than those in the low-dose and high-dose groups (all P ＜ 0.05).The percentage of cells in the G0/G1 phase of the experimental groups was lower than that of the control group and the percentage of cells in the G2/M phase was higher than that of the control group,especially in the medium-dose and high-dose groups (all P ＜ 0.05).The ratios of mitochondrial membrane potential decrease in the low-dose,medium-dose and high-dose groups and control group were 34.77％ ± 5.33％,37.87％ ± 5.73％,18.37％ ± 3.91％,and 4.17％ ± 0.65％,respectively.The mitochondrial membrane potential decreased ratios of the experimental groups were higher than that in the control group,especially in the lowdose and medium-dose groups (all P ＜ 0.05).Transmission electronic microscopy showed that the control group had normal morphology while the experimental groups had many forms of death cells,including apoptosis,necrosis and mitotic catastrophe.The apoptosis rate and mitotic catastrophe rate of the experimental groups were both higher than those of control group (both P ＜ 0.05).Conclusion Lidamycin can significantly inhibit the proliferation of Caski cells with an dose-dependent manner by inducing the apoptosis and mitotic catastrophe of cells.关键词
宫颈癌/力达霉素/人宫颈癌Caski细胞/细胞凋亡/细胞裂亡/线粒体膜电位Key words
cervical carcinoma/lidamycin/human cervical cancer Caski cells/apoptosis/mitotic catastrophe/mitochondrial membrane potential分类
医药卫生引用本文复制引用
鲁华,秦烨,占景琼,王艳林..力达霉素对人宫颈癌Caski细胞增殖的影响及其机制[J].山东医药,2017,57(44):1-4,4.基金项目
国家自然科学基金资助项目(81372265). （81372265）
