山东医药2017，Vol.57Issue(44)：9-13,5.DOI:10.3969/j.issn.1002-266X.2017.44.003
FAM92A1-289对人脑胶质瘤U251细胞增殖、迁移能力的影响及其与Galectin-1的关系
Effects of FAM92A1-289 on proliferation and migration of glioma cells U251 and its relationship with galectin-1
摘要
Abstract
Objective To explore the function of FAM92A1-289 in regulating the proliferation and migration of glioma cells U251 and its relationship with galectin-1.Methods We transfected the plasmid CMV-SP6-TALEN-GFP-289 into U251 cells,and used puromycin to get the stable cell line U251/289 ++ with over-expression of FAM92A1-289 gene.We took U251/289++ cells as the observation group,and U251 cells without transfection as the control group.The real-time cell analysis (RTCA) technology was used to detect the cell proliferation ability of the two groups (expressed by the optical density value) at 24,48,72,96,and 120 h;Transwell test was used to detect the cell migration ability of the two groups (expressed by the number of cells crossing the trans-well chamber).We constructed the PCS2-3Flag-Galectin-1 plasmid,and the U251 cells were randomly divided into FAM92A1-289 group,the Galectin-1 group,co-transfection group,and control group.In the FAM92A1-289 group and Galectin-1 group,we used the Lipofactamine 3000 transfection method to transfect CMV-SP6-TALEN-GFP-289 and PCS2-3Flag-Galectin-1 plasmids,respectively.The cells in the co-transfection group were transfected with the two plasmids,and in the control group we only added Lipofactamine 3000 transfection reagent.The interaction between FAM92A1-289 and Galectin-1 protein was verified by Co-Immunoprecipitation.Results There was no statistically significant difference between the two groups cells at 24 h (P ＞ 0.05).The optical density value at 48,72,96 and 120 h was significantly higher than that in the control group (P ＜ 0.05 or P ＜ 0.01).The number of cells crossing the chamber in the observation group and the control group were 242.0 ± 11.41 and 65.40 ± 7.92,respectively (P ＜ 0.01).The relative expression of GFP in co-transfection group was significantly higher than that in the FAM92A1-289 group,Galectin-1 group,and control group (P ＜ 0.01).Conclusion The over-expression of FAM92A1-289 can improve the proliferation and migration of U251 cells,and the interaction between FAM92A1-289 and galectin-1 is used to provide evidence for the role of FAM92A1-289 in regulating the malignant behavior of glioma cells.关键词
脑胶质瘤/U251细胞/FAM92A1-289/半乳糖凝集素1/细胞增殖/细胞迁移Key words
glioma/U251 cells/FAM92A1-289/galectin-1/cell proliferation/cell migration分类
医药卫生引用本文复制引用
吴慕禹,郭兴荣,李东升..FAM92A1-289对人脑胶质瘤U251细胞增殖、迁移能力的影响及其与Galectin-1的关系[J].山东医药,2017,57(44):9-13,5.基金项目
国家自然科学基金应急管理项目(81641028) （81641028）
湖北省自然科学基金面上项目(2016CFB408). （2016CFB408）
