山东医药2017，Vol.57Issue(44)：14-17,4.DOI:10.3969/j.issn.1002-266X.2017.44.004
独活寄生汤含药血清对膝关节OA大鼠滑膜细胞TNF-α、IL-6、IL-8、IL-10表达的影响及其机制
Effect of Duhuo Jisheng decoction drug-containing serum on expression of synovial cell TNA-α, IL-6, IL-8, IL-10 in knee osteoarthritis rats
摘要
Abstract
Objective To observe the effect of Duhuo Jisheng decoction drug-containing serum on the expression of inflammatory factors in synovial cells of knee osteoarthritis (OA) rats and to explore its possible mechanism.Methods The rat model of OA was established by Hulth method.The synovial cells of knee joint were cultured by enzymatic digestion.The synovial cells were divided into the model group and low-dose,medium-dose and high-dose groups.The normal rat synovial cells were used as the control group.The rats in the low-dose,medium-dose and high-dose groups were added with the serum containing Duhuo Jisheng decoction 0.85,1.7 and 3.4 mL/kg by gavage,respectively,while rats in the control group and the model group were given the same amount of 20％ fetal bovine serum,and then both of them were cultured for 48 h.The levels of TNF-α,IL-6,IL-8,and IL-10 were detected by ELISA.The expression of lipoxin A4 was detected by high performance liquid chromatography-high resolution mass spectrometry.Western blotting was used to detect the expression of Anti-c-Jun amino terminal kinase (JNK),p-JNK,activator protein 1 (AP-1),and p-AP-1 expression.Results The expression levels of TNF-α,IL-6,and IL-8 in the control group,high-dose group,mediumdose group,low-dose group,and model group gradually increased,the expression levels of IL-10 and lipoxin A4 gradually decreased,and significant difference was found between every two groups (all P ＜ 0.05).Compared with the control group,the expression levels of AP-1,p-AP-1,JNK,and p-JNK in the model group increased (all P ＜ 0.05).Compared with the model group,the expression levels of AP-1,p-AP-1,JNK,and p-JNK gradually decreased in the high-dose,medium-dose and low-dose groups,and the decrease was more significant in the high-dose group (all P ＜ 0.05).Conclusion Duhuo Jisheng decoction can reduce the expression of TNA-α,'IL-6,IL-8,IL-10 in the synovial cells of knee OA rats in a dose-dependent manner by inhibiting JNK,AP-1 and its phosphorylation pathway and increasing the expression of lipoxin A4 in synovial cells.关键词
骨关节炎/膝关节/独活寄生汤/滑膜细胞/脂氧素A4/炎症反应/大鼠Key words
osteoarthritis/knee/Duhuo Jisheng decoction/synovial cells/lipoxy A4/inflammatory response/rats分类
医药卫生引用本文复制引用
杜津莉,樊炜骏,李建,陈少青,孙鹏..独活寄生汤含药血清对膝关节OA大鼠滑膜细胞TNF-α、IL-6、IL-8、IL-10表达的影响及其机制[J].山东医药,2017,57(44):14-17,4.基金项目
内蒙古自治区自然科学基金资助项目(2016MS08125). （2016MS08125）
