山东医药2017，Vol.57Issue(44)：22-25,4.DOI:10.3969/j.issn.1002-266X.2017.44.006
miR-106b在非小细胞肺癌细胞A549中的表达变化及意义
Expression changes of miR-106b in non-small-cell lung cancer cell line A549
摘要
Abstract
Objective To observe the expression changes of miR-106b in non-small-cell lung cancer (NSCLC) cell line A549,and to investigate its effects on cell migration and invasion,as well as the regulatory mechanism.Methods The expression level of miR-106b was measured in A549 and HPL-1 cells by the fluorescent quantitative PCR.A549 cells were divided into three groups.The miR-106b mimics group was transfected with miR-106b mimics,the miR-106b inhibition group with miR-106b inhibit,and the control group with scramble.The relative expression level of miR-106b was measured by real-time fluorescent quantitative PCR (qRT-PCR) at 24 h after culture.The migration and invasion abilities in the three groups were measured by Scratch test and Transwell invasion assay.The expression levels of FUT6 mRNA and protein were measured in these three groups by fluorescent quantitative PCR and Western blotting,respectively.Results The expression level of miR-106b in the A549 cells was 5.37 ± 0.42,which was higher than that of HPL-1 cells (1.0),P ＜0.01.The expression level of miR-106b was 8.94 ±0.73 in the miR-106b mimics group,0.21 ±0.03 in the miR-106b inhibit group and 1.0 in the control group,respectively,and the difference was significant,all P ＜ 0.05.The wound healing rate and the number of invasive cells in the miR-106b mimics group and control group were significantly higher than those of the miR-106 inhibition group,while the expression levels of FUT6 mRNA and protein were significantly lower than those in the miR-106b inhibit group,and the'change in the miR-106b mimics group was more significance (all P ＜ 0.05).Conclusion The miR-106b is up-regulated in the NSCLC A549 cells and the over-expression of miR-106b promotes the migration and invasion of NSCLC by down-regulating FUT6 expression.关键词
非小细胞肺癌/微小RNA-106b/细胞迁移/细胞侵袭/墨角藻糖基转移酶6Key words
non-small-cell lung cancer/microRNA-106b/cell migration/cell invasion/fucosyltransferase Ⅵ分类
医药卫生引用本文复制引用
李磊,倪正义,汤中文,周密..miR-106b在非小细胞肺癌细胞A549中的表达变化及意义[J].山东医药,2017,57(44):22-25,4.基金项目
武汉市应用基础研究计划项目. （）
