山东医药2017，Vol.57Issue(44)：26-28,3.DOI:10.3969/j.issn.1002-266X.2017.44.007
VEGF-C在口腔颌面部淋巴管瘤组织中表达变化及其对人脐静脉内皮细胞增殖的影响
Expression of VEGF-C in oral and maxillofacial lymphangioma and its effect on proliferation of human umbilical vein endothelial cells
摘要
Abstract
Objective To explore the expression of vascular endothelial growth factor-C (VEGF-C) in the occurrence and development of oral and maxillofacial lymphangioma (OML).Methods The tumor tissues (OML group) and the adjacent normal tissue (control group) from OML patients were collected.The real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of VEGF-C mRNA in the tissues.Immunohistochemistry (IHC) was used to detect the positive rate of VEGF-C protein expression in the tissues.ECV304 cells cultured in vitro were randomly divided into two groups:the observation group which was transfected with sh-VEGF-C plasmid and the control group transfected with control plasmid sh-control.After 48-hour transfection,qRT-PCR and Western blotting were used to detect the relative expression of VEGF-C protein,and MTT assay was used to detect the proliferation of the cells (absorbance value).Results In the OML group and control group,the expression of VEGF-C mRNA was 1.061 ± 0.284 and 0.801 ± 0.098,respectively;the positive expression rates of VEGF-C protein was 84.10％ (42/54) and 10.13％ (5/54),respectively (P ＜ 0.05 or P ＜ 0.01).In the observation group and control group,the relative expression levels of VEGF-C mRNA were 0.200 ±0.058 and 0.967 ±0.017,respectively;the relative expression levels of VEGF-C protein were 0.319 ±0.021 and 0.965 ± 0.039,respectively;the A values were 0.393 ± 0.015 and 0.97 ± 0.004,respectively;significant differences were found between these two groups (all P ＜ 0.01).Conclusions VEGF-C is highly expressed in the OML tissues,and decreasing VEGF-C can inhibit the proliferation of human umbilical vein endothelial cells.关键词
口腔颌面部淋巴管瘤/血管内皮生长因子C/人脐静脉内皮细胞/细胞增殖Key words
oral and maxillofacial lymphangioma/vascular endothelial growth factor-C/human umbilical vein endothelial cells/cell proliferation分类
医药卫生引用本文复制引用
王国云,杨旭,徐天舒..VEGF-C在口腔颌面部淋巴管瘤组织中表达变化及其对人脐静脉内皮细胞增殖的影响[J].山东医药,2017,57(44):26-28,3.基金项目
江苏省科学技术奖资助项目(2016562). （2016562）
