山东医药2018，Vol.58Issue(17)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.17.001
流体剪切应力对氧化型低密度脂蛋白诱导血管内皮细胞损伤及黏着斑重塑的影响
Effects of fluid shear stress on vascular endothelial cell injury and focal adhesion remodeling induced by oxidized low-density lipoprotein
摘要
Abstract
Objective To investigate the effects of fluid shear stress on the vascular endothelial cell damage and focal adhesion remodeling induced by oxidized low-density lipoprotein(ox-LDL).Methods Human umbilical vein endothelial cells(HUVECs)injury was induced by 100 μg/mL ox-LDL and then we constructed the 5 and 25 dynes/cm2fluid shear stress.The cells were divided into the static group, static plus ox-LDL intervention group,5 dyne/cm2fluid shear stress plus ox-LDL intervention group,and 25 dyne/cm2fluid shear stress plus ox-LDL intervention group.The morphology of the cells was observed by phase contrast microscopy,and the retention rate of the cells was measured.The expression levels of major components of focal adhesions including Integrin-β1,focal adhesion kinase(FAK),and Paxillin were measured by immunofluorescence.Results The morphology of cells in the static group was normal.The endothelial cells in the static plus ox-LDL intervention group lost their typical morphology,with disorder arrangement and shedding.The morphology of cells in the 5 dyne/cm2fluid shear stress plus ox-LDL intervention group was improved, the shedding decreased, but the arrangement was in disorder.The cells in the 25 dyne/cm2fluid shear stress plus ox-LDL intervention group were arranged closely,with less shedding,and more solid adhesion of cell-to-matrix.The cell retention rate of 25 dyne/cm2fluid shear stress plus ox-LDL intervention group was 95.15%±16.20 %,which was higher than that of the static plus ox-LDL group (80.95%±14.72 %)and 5 dyne/cm2fluid shear stress plus LDL intervention group(88.86%±14.23%)(both P<0.05).The fluorescence intensity of Integrin-β1,FAK,and Paxillin in the static plus ox-LDL intervention group,5 and 25 dyne/cm2fluid shear stress plus ox-LDL intervention groups was higher than that of static group(all P<0.05).The fluo-rescence intensity of Integrin-β1,FAK,and Paxillin in the 5 dyne/cm2fluid shear stress plus ox-LDL intervention group was lower than that of the static plus ox-LDL intervention group(all P<0.05).The fluorescence intensity of Integrin-β1, FAK,and Paxillin in the 25 dyne/cm2fluid shear stress plus ox-LDL intervention group was lower than that of the static plus ox-LDL intervention group and 5 dyne/cm2fluid shear stress plus ox-LDL intervention group(all P<0.05).Conclu-sions The ox-LDL can cause vascular endothelial cell injury, shedding, and remodeling of focal adhesions.However, higher levels of laminar shear stress in the physiological range can effectively reduce excessive remodeling of focal adhe -sions,promote adhesion of vascular endothelial cells to matrix,and reduce vascular endothelial cell injury.关键词
血管内皮细胞损伤/流体剪切应力/氧化型低密度脂蛋白/黏着斑Key words
vascular endothelial cell injury/fluid shear stress/oxidized low-density lipoprotein/focal adhesion分类
医药卫生引用本文复制引用
钟挺挺,李燕玲,何小洪,董吁钢,马虹,郑振声,张焰..流体剪切应力对氧化型低密度脂蛋白诱导血管内皮细胞损伤及黏着斑重塑的影响[J].山东医药,2018,58(17):1-4,4.基金项目
国家自然科学基金资助项目(30871047). （30871047）
