山东医药2018，Vol.58Issue(17)：5-8,4.DOI:10.3969/j.issn.1002-266X.2018.17.002
甘蔗叶多糖对心肌梗死大鼠心肌细胞凋亡的抑制作用及其机制
Inhibitory effects of sugarcane leaf polysaccharides on cardiomyocyte apoptosis in rats with myocardial infarction
摘要
Abstract
Objective To investigate the inhibitory effects of polysaccharide from sugarcane leaves on the cardiomyo -cyte apoptosis in rats withmyocardial infarction(MI).Methods We established the MI models by ligating the left anterior descending(LAD)of rats, and then 30 rats were randomly divided into themodel control group(MI group), sugarcane leaf polysaccharide group(SLP group), in addition, sham operation group was set, however, the rats in the sham group were only threaded without ligation.The rats in the SLP group were fed with 4 mg/mL SLP solution,and the rats in the MI group and sham group were fed with the same volume of normal saline.After 4 weeks,the rat's heart was obtained by thora-cotomy,we measured the myocardial apoptosis in the infarct marginal zone by TUNEL and performed HE staining,then we detected the gene expression of BCL-2,BAX,and Caspase3 by real-time PCR,detected the protein expression of p-AKT/AKT and p-PI3K /PI3K at the edge of myocardial infraction by Western blotting.Results The apoptosis in rats of the sham group was not detected,but was detected in the marginal zone of the MI group and SLP group.The apoptotic index of the sham group,MI group,and SLP group were 0.017%±0.007%,0.307%±0.048%,and 0.109%±0.034%, re-spectively.There was significant difference in the apoptotic index of myocardial cells between every two groups(P <0.05).Compared with the sham group.myocardial apoptosis index increased in the MI group and SLP group,but myocar-dial apoptosis index in the SLP group was lower than that in the MI group(all P<0.05).Compared with the sham group, BAX,BCL-2,and Caspase-3 increased in the MI group(P<0.05).Compared with the MI group,the expression of BCL-2 mRNA increased,while the expression of BAX and Caspase-3 mRNA decreased in the SLP group(all P<0.05).Com-pared with the sham group, the protein expression of p-AKT/AKT and p-PI3K/PI3K decreased in the MI group(P<0.05).Compared with the MI group,the expression of p-AKT/AKT and p-PI3K/PI3K increased in the SLP group(both P<0.05).Conclusions SLP could significantly inhibit the apoptosis of myocardial cells after MI,and its mechanism may be that SLP may increase the expression of the anti-apoptosis gene BCL-2, decrease the expression of pro-apoptosis gene BAX,Caspase-3,and up-regulate the expression of p-AKT/AKT and p-PI3K/PI3K protein.关键词
心肌梗死/甘蔗叶多糖/细胞凋亡/大鼠Key words
myocardial infarction/sugarcane leaf polysaccharide/apoptosis/rats分类
医药卫生引用本文复制引用
刘丹,林锟,侯小涛,秦超,何涛..甘蔗叶多糖对心肌梗死大鼠心肌细胞凋亡的抑制作用及其机制[J].山东医药,2018,58(17):5-8,4.基金项目
国家自然科学基金资助项目(81360655) （81360655）
广西自然科学基金资助项目(2010GXNSFA013173). （2010GXNSFA013173）
