实验动物与比较医学2017，Vol.37Issue(5)：337-343,7.DOI:10.3969/j.issn.1674-5817.2017.05.001
体外诱导树鼩骨髓间充质干细胞向神经元样细胞分化的实验研究
Induction and Differentiation of Tree Shrew Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells in Vitro
摘要
Abstract
Objective To observe the feasibility of the differentiation of tree shrew (Tupaia belangeri) bone marrow mesenchymal stem cells (BM-MSCs) to neuron-like cells.Method The tree-shrew BM-MSCs were differentiated to neuron-like cells by IMEM differentiation culture mediums (with different inducer and concentration).Identify the secretion concentration of neuron-specific enolase (NES) and α-synuclein (α-SYN) by PCR.NES antibody was also used to identify cell immunofluorescence.Result The result of the tree-shrew BM-MSCs differentiation to neuron-like cells with IMEM cell culture differentiation mediums (10 μg/mL EGF and 20 μg/mL bFGF) were good on 12d in-vitro.Conclusion Optimized cnlture medium with double grow factors and differentiation method were more appropriated for tree shrew BM-MSCs differentiation to neuron-like cells,and the cells differentiated by this manner were consistent to neuron-like cells characteristics.关键词
树鼩/间充质干细胞(MSCs)/体外诱导培养/神经元样细胞Key words
Tree shrew (Tupaia belangeri)/Bone marrow mesenchymal stem cells (BM-MSCs)/Neuron-like cells分类
生物科学引用本文复制引用
苗雨润,李娜,匡德宣,宋庆凯,袁圆,王璇,张志成,陆彩霞..体外诱导树鼩骨髓间充质干细胞向神经元样细胞分化的实验研究[J].实验动物与比较医学,2017,37(5):337-343,7.基金项目
国家自然科学基金项目(No.31601907) （No.31601907）
实验树鼩标准化与应用研究云南省创新团队 （）
云南省联合支持国家计划项目(No.2015GA009) （No.2015GA009）
