实验动物与比较医学2017，Vol.37Issue(5)：344-351,8.DOI:10.3969/j.issn.1674-5817.2017.05.002
瘦素基因敲除小鼠模型的建立及表型分析
Generation and Phenotyping Analysis of Lep Gene Knockout Mice
摘要
Abstract
Objective To provide mouse models for obesity and diabetes research and drug development,the Leptin (Lep) gene knockout mouse model was established and analyzed.Methods The Lep gene knockout mouse model was established by using embryonic stem cell and homologus recombination technology.Body weight and plasma glucose were measured,and the three genotypes of Lep gene knockout mice were sacrificed for detection of the serum biochemistry parameters.All the data generated from Lep gene knockout mice were compared with age-matched heterozygote(He) and wild type (WT) mice in statistics.Pathological changes of hepatic and kidney tissues between Lep gene knockout and WT mice were observed under microscope.Results The Lep gene knockout mouse model was established.Preliminarily phenotypic analysis show that Lep gene knockout mice develop obesity and hyperinsulinemia,hyperglycemia,lipid metabolism disorder,liver dysfunction.From the pathological analysis,the hepatocyte swelling and macro vacuolar steatosis were observed.Conclusion The Lep gene knockout mouse model was established and it could be used for obesity and diabetes research and drug development.关键词
瘦素(Lep)基因/基因敲除小鼠/肥胖/Ⅱ型糖尿病Key words
Leptin (Lep) gene/Gene knockout mice/Obesity/Type 2 Diabetes分类
生物科学引用本文复制引用
池骏,龚慧,何玥炜,万颖寒,费俭,匡颖..瘦素基因敲除小鼠模型的建立及表型分析[J].实验动物与比较医学,2017,37(5):344-351,8.基金项目
上海市科委项目资助(14140900400,15DZ2290800) （14140900400,15DZ2290800）
