实验动物与比较医学2017，Vol.37Issue(5)：357-362,6.DOI:10.3969/j.issn.1674-5817.2017.05.004
大鼠离体肾脏灌流模型在筛选慢性肾病药物的初步应用
Pilot Application of Isolated Perfused Chronic Kidney Transgenic Rat Kidney Model for Discovery and Evaluation of Chronic Kidney Disease Drug
傅婷婷 1刘艳2
作者信息
- 1. 复旦大学生命科学学院,上海200438
- 2. 阿斯利康中国创新中心,上海201203
- 折叠
摘要
Abstract
Objective To establish isolated perfused kidney (IPK) model in chronic kidney disease (CKD) transgenic rat,as a screening tool for CKD drug.Method Seven CKD transgenic rats were perfused with 1 μmol/L CysA for 60 minutes,the content of albumin in urine were detected before and after treatment to evaluate the effect of CysA.Five CKD transgenic rats were treated with 20 mg/kg CysA/day for 15 days,the content of albumin in urine were compared to 5 control CKD transgenic rats to evaluate in vivo effect of CysA.Results After perfusing 1 μmol/L CysA for 60 minutes,the content of albumin in urine were decrease signicantly.In in vivo efficacy study,after dosing CysA 15days,the content of albumin in urine were also decrease signicantly.Conclusion The consistency of CysA efficacy of decreasing the content of albumin in urine bewteen IPK model and in vivo animal model in CKD transgenic rat is good.The IPK model on CKD transgenic rat have potential to be a further screening tool for CKD drug.关键词
离体肾脏灌流(IPK)/环孢素A(CysA)/慢性肾病(CKD)Key words
Isolated perfused kidney(IPK)/Cyclosporin A (CysA)/Chronic kidney disease (CKD)分类
生物科学引用本文复制引用
傅婷婷,刘艳..大鼠离体肾脏灌流模型在筛选慢性肾病药物的初步应用[J].实验动物与比较医学,2017,37(5):357-362,6.
