实验动物与比较医学2017，Vol.37Issue(5)：383-389,7.DOI:10.3969/j.issn.1674-5817.2017.05.008
应用无约束全身体积描记系统检测小鼠肺功能
Measurement of Lung Function by Using Non-invasive Whole-body Plethysmograph System in Mice
摘要
Abstract
Objective To establish a method for testing pulmonary function by Buxco's non-invasive whole-body plethysmography (WBP) in mice.Methods Twenty female ICR mice were randomly divided into control group and asthmatic model group.Each mouse in model group was subcutaneously injected (multisites) and intraperitoneally injected (one site) with ovalbumin (OVA) which was dissolved in aluminium hydroxide adjuvant.OVA-sensitized mice were challenged by inhalation of antigen.For bronchial provocation test,various concentration of methacholine (Mch) aerosolized by a jet nebulizer.Respiration parameters and lung resistance,showed as the value of enhanced pause (Penh) were measured by the WBP before and after Mch challenge.Results Compared with control group,bronchial challenge of Mch dose-dependently induced significant deceleration of frequency of breathing (F) and increases of inspiration time (Ti),expiration time (Te) and the value of Penh in OVA-sensitized mice.Conclusions The results proved that the WBP of pulmonary function presented a dose-dependent on testing Mch-challenged airway hyperresponsiveness (AHR) in mice.It may be a potential testing method for AHR.关键词
小鼠/肺功能/哮喘/气道高反应性(AHR)/无创肺功能仪/无约束全身体积描记Key words
Mice/Pulmonary function/Asthma/Airway hyperresponsiveness/Non-invasive lung function detector/Whole-body plethysmography分类
生物科学引用本文复制引用
赵玮,俞冰,张水娟,贾永良,谢强敏..应用无约束全身体积描记系统检测小鼠肺功能[J].实验动物与比较医学,2017,37(5):383-389,7.基金项目
浙江省公益性技术应用研究计划(实验动物)项目(No.2015C37087) （实验动物）
