实验动物与比较医学2017，Vol.37Issue(5)：390-394,5.DOI:10.3969/j.issn.1674-5817.2017.05.009
应用树鼩对硫酸乙酰肝素佐剂的安全性初步评价
Safety Evaluation on Adjuvant Heparan Sulfate in Tree Shrew
摘要
Abstract
Objective To explore the feasibility of tree shrew as an animal model for safety evaluation,through the detection of adjuvant heparan sulfate (HS) of the tree shrew skin irritation and adjuvant with Hepatitis A virus attenuated live vaccine (HepA-l) combined with immune pathological analysis of main organs on tree shrews.Methods According to the dose frequency and skin integrity,the tree shrews were randomly divided into two groups,the skin irritation test performed by tree shrew own left and right contrast.According to the vaccine and adjuvant type the tree shrews randomly divided,subcutaneous immunization.Twenty weeks after immunization,major organs are harvested to make pathological slice and perform HE staining analysis.Results The skin irritation test of adjuvant HS on tree shrews were no erythema,edema and other irritating changes of single and multiple dosing group on integrity skin or damaged skin.There is no significant pathological change between HepA-1 and HepA-1 combined with HS group on heart,liver,spleen,lung and kidney and the brain biopsy.Conclusion The experiment proved that the tree shrew as an animal model for vaccine safety evaluation is feasible,provide the basical data for future vaccine and drug evaluation platform.关键词
树鼩/硫酸乙酰肝素(HS)/甲型肝炎减毒活疫苗(HepA-1)/皮肤刺激性实验/病理分析/安全性评价Key words
Tree shrew/Heparan sulfate(HS)/Hepatitis A virus attenuated live vaccine(HepA-1)/Skin irritation test/Pathological analysis/Safety evaluation分类
生物科学引用本文复制引用
李彦涵,李建芳,孙静,乌美妮,王海璇,胡云章..应用树鼩对硫酸乙酰肝素佐剂的安全性初步评价[J].实验动物与比较医学,2017,37(5):390-394,5.基金项目
国家科技支撑计划项目(2014BAI01B01) （2014BAI01B01）
国家重点研发计划项目(2016YFC1202305) （2016YFC1202305）
