实验动物与比较医学2017，Vol.37Issue(5)：399-404,6.DOI:10.3969/j.issn.1674-5817.2017.05.011
米非司酮对Wistar大鼠窗口期子宫内膜整合素和血管内皮生长因子基因表达的影响
Effects of Mifepristone on Expression of Integrin αvβ3 and Vascular Endothelial Growth Factor in Wistar Rats at Implantation Window Phase
摘要
Abstract
Objective To investigate the effect of mifepristone on the expression of integrin αvβ3 and vascular endothelial growth factor (VEGF) gene,and the effect on ultrastructure of endometrium at implantation window phase in Wistar rats.Methods Thirty-six Wistar rats were divided to four groups,the rats of two experimental groups were orally administered with 5 mg/kg,10 mg/kg of mifepristone respectively one day after conception.The endometrium was collected 4.5 days after mate both in two experimental groups and two control groups.Ultrastructure of the endometrium were observed,by transmission electron microscope,and the expression of integrin αvβ3 and VEGF gene in endometrial were tested by RT-PCR method,meantime,the contents of estrogen and progesterone in serum were detected by ELISA technique.Results Mifepristone can inhibits the expression of integrin αvβ3,VEGF and progesterone,and increase the content of estrogen (P＞0.05).The down-regulation of the three factors and the changes of the ultrastructure of endometrium has obvious dose-response relationship with mifepristone.Conclusion The expression of integrin αvβ3 and VEGF gene were decreased differently by mifepristone as well as the content of progesterone,eventually it will affect the ultrastructure of endometrium.关键词
米非司酮/整合素(αvβ3)/血管内皮生长因子(VEGF)/雌激素/孕激素Key words
Mifepristone/Integrin αvβ3/Vascular endothelial growth factor (VEGF)/Estrogen/Progesterone分类
生物科学引用本文复制引用
唐彬,黎福荣,付新录,江国建,赵勇,张薇..米非司酮对Wistar大鼠窗口期子宫内膜整合素和血管内皮生长因子基因表达的影响[J].实验动物与比较医学,2017,37(5):399-404,6.基金项目
广东省科技基础条件建设项目(No.2012B060300015) （No.2012B060300015）
