实验动物与比较医学2017，Vol.37Issue(5)：405-409,5.DOI:10.3969/j.issn.1674-5817.2017.05.012
超促排卵后不同取卵时间对昆明小鼠卵子质量、体内成熟率和体外受精率的影响
Different Oocyte Collecting Time Impact on Quality of Oocyte,in Vivo Maturation Rate and in Vitro Fertilization Rate after Superovulation in KM Mice
摘要
Abstract
Objective To improve the quality of oocyte,in vivo maturation rate and in vitro fertilization rate in KM mice.Methods One-hundred KM mice were super-ovulated,the changes of the oocyte nucleus and the first polar body position,the perivitelline space changes,oocyte morphology,in vivo maturation rates and in vitro fertilization rates were observed and calculated.Results The oocyte quality was best after 12 h、14 h of injection with human chorionic gonadotropins (hCG),and oocyte in vivo maturation rate and in vitro fertilization rate were the highest after 13 h.Conclusion Collection of oocyte after 13 h of superovulation was the best time for in vitro fertilization of KM mouse.关键词
小鼠/超促排卵/取卵时间/卵子质量/体内成熟率/体外受精率Key words
Mice/Superovulation/Collecting oocyte time/Oocyte quality/In vivo maturation rate/In vitro fertilization rate分类
生物科学引用本文复制引用
彭礼繁,张小建,廖梅旭,李晓洁,余鲲..超促排卵后不同取卵时间对昆明小鼠卵子质量、体内成熟率和体外受精率的影响[J].实验动物与比较医学,2017,37(5):405-409,5.基金项目
四川省科技厅软科学项目(2017ZR0060) （2017ZR0060）
