实验动物与比较医学2017，Vol.37Issue(6)：421-427,7.DOI:10.3969/j.issn.1674-5817.2017.06.001
异功散对小鼠慢性病贫血的治疗作用
Therapeutic Effects of Yi Gong San Decoction on Anemia of Chronic Disease in Mouse
摘要
Abstract
Objective To observe the therapeutic effects of Yi Gong San decoction (YGS) on anemia of chronic disease (ACD) in mouse.Methods Totally 48 male 8-week-old male C57 BL/6 mice were randomly divided into blank control group,model group,intervention group.Each group was sacrificed 8 mouse after the continuous administration of 1 week or 2 weeks.The model group and intervention group intraperitoneally injected with zymosan A after 6 days of intraperitoneally injected with LPS.After 5 days of zymosan A injection,YGS (15.413 g per day) was administered to mice intragastrically in intervention group for successive 1 or 2 weeks while 0.2 mL of ultra-pure water was administered to mice intragastrically in blank control group and the model group.The mouse blood routine was measured by automatic cell counting.Colorimetric method was used to detect serum iron (SI) and unsaturated iron binding (UIBC).ELISA was used to detect ferritin (SF).The ratio of iron in liver and spleen were analyzed by software using Prussian blue staining.The positive area of Prussian blue iron was analyzed by Image-Pro Plus 6.0 software.The expression of hepcidin antimicrobial peptide (HAMP) mRNA in mouse liver was detected by RT-PCR.Results In the 1 or 2 weeks,the content of hemoglobin (HB),erythrocyte count (RBC),hematocrit (HCT) in mice was significantly increased (vs model group,P ＜0.01 or P＜0.05);Hematocrit (MCV) was not changed (P＞0.05).The SI and UIBC was increased (VS model group,P＜0.01 or P＜0.05);the SF and the ratio of iron in liver and spleen reduced significantly (VSmodel group,P＜0.01 or P＜0.05).Liver HAMP mRNA was significantly decreased (P＜0.01 or P＜0.05).Conclusion Yi Gong San decoction can improve the degree of anemia and adjust the iron metabolism disorder in mouse of anemia of chronic disease by decreasing HAMP mRNA relative expression.关键词
慢性病贫血(ACD)/异功散(YGS)/治疗/铁调素Key words
Anemia of chronic disease (ACD)/Yi Gong San decoction (YGS)/Treatment/Hepcidin分类
生物科学引用本文复制引用
郑秦,罗梅宏,姜一陵,季玉婷,夏乐敏,张爱萍,石岭,戴程怡,薛城,邹纯朴..异功散对小鼠慢性病贫血的治疗作用[J].实验动物与比较医学,2017,37(6):421-427,7.基金项目
国家自然科学基金青年项目(81102569),国家自然基金面上项目(81774271),上海卫生和计划生育委员会科研项目(201440587),上海市自然科学基金面上项目(16ZR1425400) （81102569）
