实验动物与比较医学2017，Vol.37Issue(6)：428-433,6.DOI:10.3969/j.issn.1674-5817.2017.06.002
几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用
The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice
摘要
Abstract
Objective To explore the role of inflammation in angiogenesis in choke area Methods The ear flap model of mice was established and the changes of blood vessel diameter was observed by HE staining.The expressions of intercellular cell adhesion molecule-1 (ICAM-1),monocyte chemoattractant protein-1 (MCP-1) and cluster of differentiation 68 (CD68) were detected by immunohistochemistry on the day after modeling(0 d),1 d,3 d,5 d and 7 d,respectively.Result By observing the vascular diameter of the ear flap in mice,it was found that the vessels were visible at 3 d after operation and the vascular dilatation was obvious at 7 d.CD68-1abeled macrophages had the largest accumulation in two choke area at 3 d.High expression of MCP-1 and ICAM-1 was observed in the early stage of postoperative inflammatory response.The expression of the three inflammatory factors in the tissue was increased significantly during the vascular dilation.Conclusion The three inflammatory factors (ICAM-1,CD68 and MCP-1) play an important role in vascular remodeling.Proper inflammatory response plays a positive role in flap survival.关键词
免疫组织化学/炎症因子/choke区/血管Key words
Immunohistochemistry/Inflammatory factor/Choke area/Vessels分类
生物科学引用本文复制引用
温福利,梁成,庄跃宏,马雷,郑和平..几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用[J].实验动物与比较医学,2017,37(6):428-433,6.基金项目
福建省自然科学基金资助项目(2015J01412)和全军实验动物科研课题(SYDW(2016)007) （2015J01412）
